Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells

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	Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells
	Zhang, Xiao-Hong 1,2; Zhang, Nan 3; Lu, Jian-Mei 4; Kong, Qing-Zhong 1; Zhao, Yun-Feng 1,2
	2012-03-31
发表期刊	BIOMOLECULES & THERAPEUTICS
ISSN	1976-9148
卷号	20 期号:2 页码:177-182
文章类型	Article
摘要	Tetrazolium violet is a tetrazolium salt and has been proposed as an antitumor agent. In this study, we reported for the first time that tetrazolium violet not only inhibited human lung cancer A549 cell proliferation but also induced apoptosis and blocked cell cycle progression in the G1 phase. The results showed that tetrazolium violet significantly decreased the viability of A549 cells at 5-15 mu M. Tetrazolium violet -induced apoptosis in A549 cells was confirmed by H33258 staining assay. In A549, tetrazolium violet blocked the progression of the cell cycle at G1 phase by inducing p53 expression and further up-regulating p21/WAF1 expression. In addition, an enhancement in Fas/APO-1 and its two forms of ligands, membrane-bound Fas ligand (mFasL) and soluble Fas ligand (sFasL), as well as caspase, were responsible for the apoptotic effect induced by tetrazolium violet. The conclusion of this study is that tetrazolium violet induced p53 expression which caused cell cycle arrest and apoptosis. These findings suggest that tetrazolium violet has strong potential for development as an agent for treatment lung cancer.; Tetrazolium violet is a tetrazolium salt and has been proposed as an antitumor agent. In this study, we reported for the first time that tetrazolium violet not only inhibited human lung cancer A549 cell proliferation but also induced apoptosis and blocked cell cycle progression in the G1 phase. The results showed that tetrazolium violet significantly decreased the viability of A549 cells at 5-15 mu M. Tetrazolium violet -induced apoptosis in A549 cells was confirmed by H33258 staining assay. In A549, tetrazolium violet blocked the progression of the cell cycle at G1 phase by inducing p53 expression and further up-regulating p21/WAF1 expression. In addition, an enhancement in Fas/APO-1 and its two forms of ligands, membrane-bound Fas ligand (mFasL) and soluble Fas ligand (sFasL), as well as caspase, were responsible for the apoptotic effect induced by tetrazolium violet. The conclusion of this study is that tetrazolium violet induced p53 expression which caused cell cycle arrest and apoptosis. These findings suggest that tetrazolium violet has strong potential for development as an agent for treatment lung cancer.
关键词	A549 Cell Apoptosis Fas Fas Ligand P21 P53 Tetrazolium Violet
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	UP-REGULATION ; MEDIATED APOPTOSIS ; CASPASE ACTIVATION ; TUMOR-CELLS ; HEPG2 CELLS ; FAS ; P53 ; DEATH ; PATHWAYS ; PROTEIN
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
WOS类目	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
WOS记录号	WOS:000303224300007
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/3704
专题	中国科学院西北高原生物研究所
作者单位	1.Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China 2.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810008, Peoples R China 3.Jinan Cent Hosp, Dept Med, Jinan, Shandong Provin, Peoples R China 4.Qufu Normal Univ, Sch Life Sci, Qufu 273165, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiao-Hong,Zhang, Nan,Lu, Jian-Mei,et al. Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells[J]. BIOMOLECULES & THERAPEUTICS,2012,20(2):177-182.
APA	Zhang, Xiao-Hong,Zhang, Nan,Lu, Jian-Mei,Kong, Qing-Zhong,&Zhao, Yun-Feng.(2012).Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells.BIOMOLECULES & THERAPEUTICS,20(2),177-182.
MLA	Zhang, Xiao-Hong,et al."Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells".BIOMOLECULES & THERAPEUTICS 20.2(2012):177-182.

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