Preparative separation and purification of four cis-trans isomers of coumaroylspermidine analogs from safflower by high-speed counter-current chromatography

NWIPB OpenIR

	Preparative separation and purification of four cis-trans isomers of coumaroylspermidine analogs from safflower by high-speed counter-current chromatography
	Li, Wen-Cong ; Wang, Xiao-Yan ; Lin, Peng-Cheng ; Hu, Na ; Zhang, Qiu-Long ; Suo, You-Rui ; Ding, Chen-Xu ; Ding, CX (reprint author), Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining 810001, Peoples R China.
	2013-11-01
发表期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ; Li, WC; Wang, XY; Lin, PC; Hu, N; Zhang, QL; Suo, YR; Ding, CX.Preparative separation and purification of four cis-trans isomers of coumaroylspermidine analogs from safflower by high-speed counter-current chromatography,JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2013,938():75-79
摘要	High-speed counter-current chromatography (HSCCC) was successfully applied for the first time to isolate and purify four cis-trans isomers of coumaroylspermidine analogs from Safflower. HSCCC separation was achieved with a two-phase solvent system composed of chloroform-methanol-water (1:1:1, v/v/v) with the upper phase as the mobile phase. In a single run, a total of 1.3 mg of N-1, N-5, N-10-(E)-tri-p-coumaroylspermidine (EEE), 4.4 mg of N-1(E)-N-5-(Z)-N-10-(E)-tri-p-coumaroylspermidine (EZE), 7.2 mg of N-1(Z)-N-5-(z)-N-10-(E)-tri-p-coumaroylspermidine (ZZE), and 11.5 mg of N-1,N-5,N-10-(Z)-tri-p-coumaroylspermidine (ZZZ) were obtained from 100 mg of crude sample. High Performance Liquid Chromatography (HPLC) analysis showed that the purities of these four components are 95.5%, 98.1%, 97.5% and 96.2%, respectively. The chemical structures were identified by ESI-MS, H-1 NMR and C-13 NMR. (c) 2013 Elsevier B.V. All rights reserved.; High-speed counter-current chromatography (HSCCC) was successfully applied for the first time to isolate and purify four cis-trans isomers of coumaroylspermidine analogs from Safflower. HSCCC separation was achieved with a two-phase solvent system composed of chloroform-methanol-water (1:1:1, v/v/v) with the upper phase as the mobile phase. In a single run, a total of 1.3 mg of N-1, N-5, N-10-(E)-tri-p-coumaroylspermidine (EEE), 4.4 mg of N-1(E)-N-5-(Z)-N-10-(E)-tri-p-coumaroylspermidine (EZE), 7.2 mg of N-1(Z)-N-5-(z)-N-10-(E)-tri-p-coumaroylspermidine (ZZE), and 11.5 mg of N-1,N-5,N-10-(Z)-tri-p-coumaroylspermidine (ZZZ) were obtained from 100 mg of crude sample. High Performance Liquid Chromatography (HPLC) analysis showed that the purities of these four components are 95.5%, 98.1%, 97.5% and 96.2%, respectively. The chemical structures were identified by ESI-MS, H-1 NMR and C-13 NMR. (c) 2013 Elsevier B.V. All rights reserved.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/37068
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, Wen-Cong,Wang, Xiao-Yan,Lin, Peng-Cheng,et al. Preparative separation and purification of four cis-trans isomers of coumaroylspermidine analogs from safflower by high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, Li, WC; Wang, XY; Lin, PC; Hu, N; Zhang, QL; Suo, YR; Ding, CX.Preparative separation and purification of four cis-trans isomers of coumaroylspermidine analogs from safflower by high-speed counter-current chromatography,JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2013,938():75-79,2013.
APA	Li, Wen-Cong.,Wang, Xiao-Yan.,Lin, Peng-Cheng.,Hu, Na.,Zhang, Qiu-Long.,...&Ding, CX .(2013).Preparative separation and purification of four cis-trans isomers of coumaroylspermidine analogs from safflower by high-speed counter-current chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES.
MLA	Li, Wen-Cong,et al."Preparative separation and purification of four cis-trans isomers of coumaroylspermidine analogs from safflower by high-speed counter-current chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2013).