Sensitive, accurate and rapid detection of trace aliphatic amines in environmental samples with ultrasonic-assisted derivatization microextraction using a new fluorescent reagent for high performance liquid chromatography

NWIPB OpenIR

	Sensitive, accurate and rapid detection of trace aliphatic amines in environmental samples with ultrasonic-assisted derivatization microextraction using a new fluorescent reagent for high performance liquid chromatography
	Chen, Guang ; Liu, Jianjun ; Liu, Mengge ; Li, Guoliang ; Sun, Zhiwei ; Zhang, Shijuan ; Song, Cuihua ; Wang, Hua ; Suo, Yourui ; You, Jinmao
	2014-07-25
发表期刊	JOURNAL OF CHROMATOGRAPHY A ; Chen, G; Liu, JJ; Liu, MG; Li, GL; Sun, ZW; Zhang, SJ; Song, CH; Wang, H; Suo, YR; You, JM.Sensitive, accurate and rapid detection of trace aliphatic amines in environmental samples with ultrasonic-assisted derivatization microextraction using a new fluorescent reagent for high performance liquid chromatography,JOURNAL OF CHROMATOGRAPHY A,2014,1352():8
摘要	A new fluorescent reagent, 1-(1H-imidazol-1-yl)-2-(2-pheny1-1H-phenanthro[9,10-d]imidazol-1-yl]ethanone (IPPIE), is synthesized, and a simple pretreatment based on ultrasonic-assisted derivatization microextraction (UDME) with IPPIE is proposed for the selective derivatization of 12 aliphatic amines (C-1: methylamine-C-12: dodecylamine) in complex matrix samples (irrigation water, river water, waste water, cultivated soil, riverbank soil and riverbed soil). Under the optimal experimental conditions (solvent: ACN-HCl, catalyst: none, molar ratio: 43, time: 8 min and temperature: 80 C), micro amount of sample (40 mu L; 5 mg) can be pretreated in only 10 min, with no preconcentration, evaporation or other additional manual operations required. The interfering substances (aromatic amines, aliphatic alcohols and phenols) get the derivatization yields of <5%, causing insignificant matrix effects (<4%). IPPIE-analyte derivatives are separated by high performance liquid chromatography (HPLC) and quantified by fluorescence detection (FD). The very low instrumental detection limits (IDL: 0.66-4.02 ng/L) and method detection limits (MDL: 0.04-033 ng/g; 5.96-45.61 ng/L) are achieved. Analytes are further identified from adjacent peaks by on-line ion trap mass spectrometry (MS), thereby avoiding additional operations for impurities. With this UDME-HPLC-FD-MS method, the accuracy (-0.73-2.12%), precision (intra-day: 0.87-3.39%; inter-day: 0.16-4.12%), recovery (97.01-104.10%) and sensitivity were significantly improved. Successful applications in environmental samples demonstrate the superiority of this method in the sensitive, accurate and rapid determination of trace aliphatic amines in micro amount of complex samples. (C) 2014 Elsevier B.V. All rights reserved.; A new fluorescent reagent, 1-(1H-imidazol-1-yl)-2-(2-pheny1-1H-phenanthro[9,10-d]imidazol-1-yl]ethanone (IPPIE), is synthesized, and a simple pretreatment based on ultrasonic-assisted derivatization microextraction (UDME) with IPPIE is proposed for the selective derivatization of 12 aliphatic amines (C-1: methylamine-C-12: dodecylamine) in complex matrix samples (irrigation water, river water, waste water, cultivated soil, riverbank soil and riverbed soil). Under the optimal experimental conditions (solvent: ACN-HCl, catalyst: none, molar ratio: 43, time: 8 min and temperature: 80 C), micro amount of sample (40 mu L; 5 mg) can be pretreated in only 10 min, with no preconcentration, evaporation or other additional manual operations required. The interfering substances (aromatic amines, aliphatic alcohols and phenols) get the derivatization yields of <5%, causing insignificant matrix effects (<4%). IPPIE-analyte derivatives are separated by high performance liquid chromatography (HPLC) and quantified by fluorescence detection (FD). The very low instrumental detection limits (IDL: 0.66-4.02 ng/L) and method detection limits (MDL: 0.04-033 ng/g; 5.96-45.61 ng/L) are achieved. Analytes are further identified from adjacent peaks by on-line ion trap mass spectrometry (MS), thereby avoiding additional operations for impurities. With this UDME-HPLC-FD-MS method, the accuracy (-0.73-2.12%), precision (intra-day: 0.87-3.39%; inter-day: 0.16-4.12%), recovery (97.01-104.10%) and sensitivity were significantly improved. Successful applications in environmental samples demonstrate the superiority of this method in the sensitive, accurate and rapid determination of trace aliphatic amines in micro amount of complex samples. (C) 2014 Elsevier B.V. All rights reserved.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/37360
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chen, Guang,Liu, Jianjun,Liu, Mengge,et al. Sensitive, accurate and rapid detection of trace aliphatic amines in environmental samples with ultrasonic-assisted derivatization microextraction using a new fluorescent reagent for high performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A, Chen, G; Liu, JJ; Liu, MG; Li, GL; Sun, ZW; Zhang, SJ; Song, CH; Wang, H; Suo, YR; You, JM.Sensitive, accurate and rapid detection of trace aliphatic amines in environmental samples with ultrasonic-assisted derivatization microextraction using a new fluorescent reagent for high performance liquid chromatography,JOURNAL OF CHROMATOGRAPHY A,2014,1352():8,2014.
APA	Chen, Guang.,Liu, Jianjun.,Liu, Mengge.,Li, Guoliang.,Sun, Zhiwei.,...&You, Jinmao.(2014).Sensitive, accurate and rapid detection of trace aliphatic amines in environmental samples with ultrasonic-assisted derivatization microextraction using a new fluorescent reagent for high performance liquid chromatography.JOURNAL OF CHROMATOGRAPHY A.
MLA	Chen, Guang,et al."Sensitive, accurate and rapid detection of trace aliphatic amines in environmental samples with ultrasonic-assisted derivatization microextraction using a new fluorescent reagent for high performance liquid chromatography".JOURNAL OF CHROMATOGRAPHY A (2014).