Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography

NWIPB OpenIR

	Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography
	Chi, Xiaofeng ; Zhang, Guoying ; Xiao, Yuancan ; Dong, Qi ; Hu, Fengzu
	2014-04-01
发表期刊	ASIAN JOURNAL OF CHEMISTRY ; Chi, XF; Zhang, GY; Xiao, YC; Dong, Q; Hu, FZ.Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography,ASIAN JOURNAL OF CHEMISTRY,2014,26(7):1905
摘要	Dispersive liquid-liquid microextraction combined with high-performance liquid chromatography-diode array detection has been successfully developed for the extraction and determination of sulfonamides in honey samples. The effects of parameters such as the, nature and volume of extraction solvent and dispersive solvent, extraction time, ionic strength and solution pH were investigated. In this 1 method, 1 nit of acetonitrile (as dispersive solvent) containing 40-mu L of 1,1,2,2-tetrachloroethane (as extraction solution) was rapidly injected into a 5 mL honey sample containing the analytes; thereby forming a cloudy solution. After extraction, phase separation was pertained by centrifugation and the enriched analytes in the sedimented,phase Were determined by HPLC-DAD. Under the optimal conditions, the detection limit of the method was 0.8-1.7 mu g kg(-1) and the relative standard deviations (RSD %) for determination of the sulfonamides were in the range of 0.172-1.549 %. Linearity was found to be in the range of 0.1-10.0 mu g mL(-1), also, the enrichment-factors, were in the range of 42-81. Finally, the method was applied to determine the trace amounts of the sulfonamides in real honey sample and satisfactory results Were obtained.; Dispersive liquid-liquid microextraction combined with high-performance liquid chromatography-diode array detection has been successfully developed for the extraction and determination of sulfonamides in honey samples. The effects of parameters such as the, nature and volume of extraction solvent and dispersive solvent, extraction time, ionic strength and solution pH were investigated. In this 1 method, 1 nit of acetonitrile (as dispersive solvent) containing 40-mu L of 1,1,2,2-tetrachloroethane (as extraction solution) was rapidly injected into a 5 mL honey sample containing the analytes; thereby forming a cloudy solution. After extraction, phase separation was pertained by centrifugation and the enriched analytes in the sedimented,phase Were determined by HPLC-DAD. Under the optimal conditions, the detection limit of the method was 0.8-1.7 mu g kg(-1) and the relative standard deviations (RSD %) for determination of the sulfonamides were in the range of 0.172-1.549 %. Linearity was found to be in the range of 0.1-10.0 mu g mL(-1), also, the enrichment-factors, were in the range of 42-81. Finally, the method was applied to determine the trace amounts of the sulfonamides in real honey sample and satisfactory results Were obtained.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/37392
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chi, Xiaofeng,Zhang, Guoying,Xiao, Yuancan,et al. Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography[J]. ASIAN JOURNAL OF CHEMISTRY, Chi, XF; Zhang, GY; Xiao, YC; Dong, Q; Hu, FZ.Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography,ASIAN JOURNAL OF CHEMISTRY,2014,26(7):1905,2014.
APA	Chi, Xiaofeng,Zhang, Guoying,Xiao, Yuancan,Dong, Qi,&Hu, Fengzu.(2014).Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography.ASIAN JOURNAL OF CHEMISTRY.
MLA	Chi, Xiaofeng,et al."Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography".ASIAN JOURNAL OF CHEMISTRY (2014).