Identification and quantification of flavonoid aglycones in rape bee pollen from Qinghai-Tibetan Plateau by HPLC-DAD-APCI/MS

NWIPB OpenIR

	Identification and quantification of flavonoid aglycones in rape bee pollen from Qinghai-Tibetan Plateau by HPLC-DAD-APCI/MS
	Lv, Huanhuan ; Wang, Xiaoyan ; He, Yanfeng ; Wang, Honglun ; Suo, Yourui ; Wang, XY (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China.
	2015-03-01
发表期刊	JOURNAL OF FOOD COMPOSITION AND ANALYSIS
摘要	For identification and quantification of flavonoid aglycones in rape bee pollen (RBP) collected from the Qinghai-Tibetan Plateau, a high-performance liquid chromatography (HPLC) separation method with diode array detector (DAD) and atmospheric pressure chemical ionization/mass spectrometric (APCI/MS) detection and four extraction methods (i.e. microwave-assisted extraction, Soxhlet extraction, cold-soaked extraction, and heat reflux extraction) were developed in this study. The identification of flavonoid aglycones was based on retention time and mass spectra by comparison with standards. Results demonstrated that this method showed excellent reproducibility and correlation coefficient, and offered the detection limits of 0.77-15.50 pmol at signal-to-noise ratio of 3. Quercetin and kaempferol were presented in RBP, and microwave-assisted extraction (MAE) was superior to the other three methods in terms of efficiency, convenience and high content of quercetin (1.37 +/- 0.059 mg/g) and kaempferol (23.44 +/- 0.544 mg/g). Our work indicated that: 1) the proposed HPLC-DAD-APCl/MS was an accurate and precise analysis method to identify and quantify the flavonoid aglycones in RBP; and 2) MAE was efficient to extract flavonoids from RBP with short extraction time, low solvent consumption, and homogeneous extraction conditions. (C) 2014 Elsevier Inc. All rights reserved.; For identification and quantification of flavonoid aglycones in rape bee pollen (RBP) collected from the Qinghai-Tibetan Plateau, a high-performance liquid chromatography (HPLC) separation method with diode array detector (DAD) and atmospheric pressure chemical ionization/mass spectrometric (APCI/MS) detection and four extraction methods (i.e. microwave-assisted extraction, Soxhlet extraction, cold-soaked extraction, and heat reflux extraction) were developed in this study. The identification of flavonoid aglycones was based on retention time and mass spectra by comparison with standards. Results demonstrated that this method showed excellent reproducibility and correlation coefficient, and offered the detection limits of 0.77-15.50 pmol at signal-to-noise ratio of 3. Quercetin and kaempferol were presented in RBP, and microwave-assisted extraction (MAE) was superior to the other three methods in terms of efficiency, convenience and high content of quercetin (1.37 +/- 0.059 mg/g) and kaempferol (23.44 +/- 0.544 mg/g). Our work indicated that: 1) the proposed HPLC-DAD-APCl/MS was an accurate and precise analysis method to identify and quantify the flavonoid aglycones in RBP; and 2) MAE was efficient to extract flavonoids from RBP with short extraction time, low solvent consumption, and homogeneous extraction conditions. (C) 2014 Elsevier Inc. All rights reserved.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/37619
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Lv, Huanhuan,Wang, Xiaoyan,He, Yanfeng,et al. Identification and quantification of flavonoid aglycones in rape bee pollen from Qinghai-Tibetan Plateau by HPLC-DAD-APCI/MS[J]. JOURNAL OF FOOD COMPOSITION AND ANALYSIS,2015.
APA	Lv, Huanhuan,Wang, Xiaoyan,He, Yanfeng,Wang, Honglun,Suo, Yourui,&Wang, XY .(2015).Identification and quantification of flavonoid aglycones in rape bee pollen from Qinghai-Tibetan Plateau by HPLC-DAD-APCI/MS.JOURNAL OF FOOD COMPOSITION AND ANALYSIS.
MLA	Lv, Huanhuan,et al."Identification and quantification of flavonoid aglycones in rape bee pollen from Qinghai-Tibetan Plateau by HPLC-DAD-APCI/MS".JOURNAL OF FOOD COMPOSITION AND ANALYSIS (2015).