Ultrasound-assisted dispersive liquid-liquid microextraction method combined with HPLC-fluorescence detection for the determination of glycyrrhetinic acid in liquorice and liquorice-derived food products

NWIPB OpenIR

	Ultrasound-assisted dispersive liquid-liquid microextraction method combined with HPLC-fluorescence detection for the determination of glycyrrhetinic acid in liquorice and liquorice-derived food products
	You, Jinmao ; Wu, Hongliang ; Li, Guoliang ; Xia, Lian ; Zhao, Mei ; Lu, Shuaimin ; Kong, Weiheng
	2016-02-01
发表期刊	JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
摘要	A sensitive and inexpensive method involving ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) and pre-column derivatization followed by high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was developed for the analysis of glycyrrhetinic acid. In this work, glycyrrhetinic acid could be obtained by hydrolyzing glycyrrhizic acid to remove glucuronic acid and subsequently extracted by UA-DLLME using chloroform and acetone as the extraction and disperser solvents, respectively. The sample extraction was firstly concentrated to dry under nitrogen and then rapidly derivatized with 2-(12-oxobenzo[b]acridin-5(12H)-yl)-ethyl-4-toluenesulfonate (BAETS) after the UA-DLLME. The prime parameters influencing the UA-DLLME and derivatization procedure were optimized using response surface methodology. Under the optimum conditions, the proposed method has a better linearity in a wider range of 6-300 ng mL(-1) and a high square of correlation coefficient (R (2)) at 0.9994. Limit of detection and limit of quantification were found to be 1.7 ng mL(-1) and 5.8 ng mL(-1), respectively. The proposed method was applied to the analysis of glycyrrhetinic acid in liquorice, liquorice apricot and sugar plum samples. For the analysis of the spiked samples, the spiked recoveries were in the range of 90.4-103.0 % with RSD less than 5.18 %. All results demonstrated that the UA-DLLME-HPLC-FLD (ultrasound-assisted dispersive liquid-liquid microextraction-high-performance liquid chromatography with fluorescence detection) was a sensitive, accurate, efficient analytical method for the determination of glycyrrhetinic acid.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/38058
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	You, Jinmao,Wu, Hongliang,Li, Guoliang,et al. Ultrasound-assisted dispersive liquid-liquid microextraction method combined with HPLC-fluorescence detection for the determination of glycyrrhetinic acid in liquorice and liquorice-derived food products[J]. JOURNAL OF THE IRANIAN CHEMICAL SOCIETY,2016.
APA	You, Jinmao.,Wu, Hongliang.,Li, Guoliang.,Xia, Lian.,Zhao, Mei.,...&Kong, Weiheng.(2016).Ultrasound-assisted dispersive liquid-liquid microextraction method combined with HPLC-fluorescence detection for the determination of glycyrrhetinic acid in liquorice and liquorice-derived food products.JOURNAL OF THE IRANIAN CHEMICAL SOCIETY.
MLA	You, Jinmao,et al."Ultrasound-assisted dispersive liquid-liquid microextraction method combined with HPLC-fluorescence detection for the determination of glycyrrhetinic acid in liquorice and liquorice-derived food products".JOURNAL OF THE IRANIAN CHEMICAL SOCIETY (2016).