NWIPB OpenIR
Fluorimetric evaluation of glutathione reductase activity and its inhibitors using carbon quantum dots
Zhu, Shuyun ; He, Lei ; Zhang, Fang ; Li, Mengyuan ; Jiao, Shulin ; Li, Yan ; Chen, Mengyuan ; Zhao, Xian-En ; Wang, Hua
2016-12-01
发表期刊TALANTA
摘要A highly sensitive fluorimetric assay has been developed for the evaluation of glutathione reductase (GR) activity and the screening of its inhibitors by using carbon quantum dots (CQDs) as the signal reporter. The detection mechanism is based on the following facts: (1) the fluorescence of CQDs can be quenched by Hg(II) through the strong CQDs-Hg(II) coordination; (2) GR can catalyze the reduction of oxidized glutathione (GSSG) into reduced glutathione (GSH), so that the fluorescence recovery of CQDs would take place resulting from the strong GSH-Hg(II) interaction; (3) GR can lose its catalytic reduction of GSSG in the presence of its inhibitors, which will inhibit the recovery of the quenched fluorescence of CQDs. The developed CQDs-based fluorimetric method can facilitate the sensitive evaluation of GR activity in the range of 0.10-2.0 mU mL(-1) with a detection limit of 0.050 mU mL(-1). In addition, other kinds of enzymes like myoglobin, thrombin, alcohol dehyrogenase, amylase, pepsin, and trypsin could show no significant effects on the evaluation of GR activity. This work may expand the biological applications of CQDs as the fluorescent probes with low cost, easy preparation, and high photostability.
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/38171
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Zhu, Shuyun,He, Lei,Zhang, Fang,et al. Fluorimetric evaluation of glutathione reductase activity and its inhibitors using carbon quantum dots[J]. TALANTA,2016.
APA Zhu, Shuyun.,He, Lei.,Zhang, Fang.,Li, Mengyuan.,Jiao, Shulin.,...&Wang, Hua.(2016).Fluorimetric evaluation of glutathione reductase activity and its inhibitors using carbon quantum dots.TALANTA.
MLA Zhu, Shuyun,et al."Fluorimetric evaluation of glutathione reductase activity and its inhibitors using carbon quantum dots".TALANTA (2016).
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