Separation of derivatized carbohydrates by capillary zone electrophoresis

NWIPB OpenIR

	Separation of derivatized carbohydrates by capillary zone electrophoresis
	Sheng Xiao ; Ding Chen-Xu ; Liu Ling-Jun ; Soo You-Rui ; Sun Zhi-Wei ; You Jin-Mao
	2008-03-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
摘要	A simple and rapid method was developed for the separation of derivatized carbohydrates using 1-naphthyl-3-methyl-5-pyrazolone (NMP) as derivatization reagent by capillary zone electrophoresis. The detection was performed with diode array at 254 nm. A 58.5 cm x 50 mu m i.d. ( 50 cm effective length) untreated fused-silica capillary was used. To optimize the conditions, the background electrolyte concentration, buffer pH, column temperature, voltage and other factors were studied. It was found that the buffer concentration and pH value have a greater impact on the separation, but the influences of temperature, voltage and additives on resolution were not obvious. On the basis of the optimization, the optimum conditions are as follows: 55 mmol/L borate buffer (pH 9.46), voltage 22 kV, column temperature at 20 degrees C and detection at 254 nm. The samples were atmospherically introduced with an injection time of 10 s. The results indicate that 9 NMP-derivatized carbohydrates achieve baseline resolution within 18 min under the proposed conditions. The Tangufican Nitratia sample was also analyzed under the optimum conditions.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/38324
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Sheng Xiao,Ding Chen-Xu,Liu Ling-Jun,et al. Separation of derivatized carbohydrates by capillary zone electrophoresis[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008.
APA	Sheng Xiao,Ding Chen-Xu,Liu Ling-Jun,Soo You-Rui,Sun Zhi-Wei,&You Jin-Mao.(2008).Separation of derivatized carbohydrates by capillary zone electrophoresis.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY.
MLA	Sheng Xiao,et al."Separation of derivatized carbohydrates by capillary zone electrophoresis".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (2008).