Optimization of Release Conditions for Acetylated Amino Sugars from Glycoprotein with the Aid of Experimental Design and Their Sensitive Determination with HPLC

NWIPB OpenIR

	Optimization of Release Conditions for Acetylated Amino Sugars from Glycoprotein with the Aid of Experimental Design and Their Sensitive Determination with HPLC
	Xia, Lian ; Liu, Lijie ; Qu, Fengli ; Kong, Rongmei ; Li, Guoliang ; You, Jinmao
	2017-06-01
发表期刊	CHROMATOGRAPHIA
摘要	The presence or absence of acetyl groups in oligosaccharides greatly affects the biological activities of glycoproteins. Consequently, in studies on glycoproteins, sensitive and accurate methods are required for the analysis of acetylated amino sugars (AASs) are required. We report here our newly developed method for the determination of four AASs [N-acetyl-d-glucosamine (GlcNAc), N-acetyl-d-galactosamine (GalNAc), N-acetyl neuraminic acid (Neu5Ac), N-glycolyl neuraminic acid (Neu5Gc)]. This method is based on the optimization of the release of AASs from Potentilla anserina L. glycoprotein and involves modification of experimental design and the use of high-performance liquid chromatography coupled with UV detection and mass spectrometry identification. Using our optimized conditions of derivatization and separation, we successfully detected these four AAS derivatives with good precision and accuracy. This is the first report of determination of the four AASs simultaneously on the basis of its release study. The newly developed method exhibits a high potential for the analysis of AASs from various bio-samples and provides a sensitive method for further study of the relationship between AAS composition and their bioactivities in glycoprotein.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/38404
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Xia, Lian,Liu, Lijie,Qu, Fengli,et al. Optimization of Release Conditions for Acetylated Amino Sugars from Glycoprotein with the Aid of Experimental Design and Their Sensitive Determination with HPLC[J]. CHROMATOGRAPHIA,2017.
APA	Xia, Lian,Liu, Lijie,Qu, Fengli,Kong, Rongmei,Li, Guoliang,&You, Jinmao.(2017).Optimization of Release Conditions for Acetylated Amino Sugars from Glycoprotein with the Aid of Experimental Design and Their Sensitive Determination with HPLC.CHROMATOGRAPHIA.
MLA	Xia, Lian,et al."Optimization of Release Conditions for Acetylated Amino Sugars from Glycoprotein with the Aid of Experimental Design and Their Sensitive Determination with HPLC".CHROMATOGRAPHIA (2017).