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A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine
Qu, Fei ; Liu, Yining ; Kong, Rongmei ; You, Jinmao
2017-11-01
发表期刊MICROCHIMICA ACTA
摘要The authors report that the fluorescence of polymer-capped silver nanoclusters (AgNCs) is quenched by MoS2 nanosheets. However, in the presence of electrolytes such as sodium acetate (50 mM), the nanosheets tend to aggregate, and this causes fluorescence to be restored. If, however, ssDNA is added, it will be adsorbed on the surface of the MoS2 nanosheets and stabilize them against aggregation. As a result, the emission of the AgNCs (best measured at excitation/emission wavelengths of 375/455 nm) is quenched again. If complementary oligonucleotide is added, a dsDNA will be formed that does no longer prevent aggregation and fluorescence is restored. Similarly, if an aptamer is adsorbed on the MoS2 nanosheets and its substrate is then added to form a folded state, the fluorescence of the AgNCs also will be restored. As a proof of concept, the DNA sequences of hepatitis B virus and of a dopamine-selective aptamer were chosen as models. The resulting assays have low detection limits and linear ranges that extend from 5 to 30 nM for hepatitis B DNA, and from 0.3 to 1 mu M for dopamine. In our perception, this method represents a universal platform for the detection of analytes for which complementary oligomers or aptamers are available.
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/38496
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Qu, Fei,Liu, Yining,Kong, Rongmei,et al. A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine[J]. MICROCHIMICA ACTA,2017.
APA Qu, Fei,Liu, Yining,Kong, Rongmei,&You, Jinmao.(2017).A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine.MICROCHIMICA ACTA.
MLA Qu, Fei,et al."A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine".MICROCHIMICA ACTA (2017).
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