NWIPB OpenIR
青海栽培何首乌中三种抗氧化活性芪多酚类物质的筛选、分离制备方法
迟晓峰 ; 胡风祖 ; 陈世龙 ; 董琦 ; 肖远灿
2016-03-09
Abstract青海栽培何首乌中三种抗氧化活性芪多酚类物质的筛选、分离制备方法,包括以下步骤:将粉碎的何首乌的根部用酒精进行热回流提取,合并得提取液;提取液经减压蒸干至恒重,得何首乌提取物;何首乌提取物用去离子水溶解后依次用石油醚、乙酸乙酯、正丁醇萃取,得到四部分萃取物;萃取物用甲醇溶解后,采用在线抗氧化筛选法筛选出正丁醇萃取物中有三种抗氧化活性成分;正丁醇萃取物溶于水并上AB-8大孔树脂柱洗脱得到含有芪多酚类物质的洗脱液;洗脱液蒸干至恒重得芪多酚粗提物;芪多酚粗提物采用高速逆流色谱设备,在氯仿-正丁醇-甲醇-水体系中进行分离,即可分别得到反式白藜芦醇-3-0-葡萄糖苷、pieceid-2"-0-gallate和反式白藜芦醇。本发明简便、快速、有效、成本低廉。
Document Type专利
Identifierhttp://210.75.249.4/handle/363003/38672
Collection中国科学院西北高原生物研究所
Recommended Citation
GB/T 7714
迟晓峰,胡风祖,陈世龙,等. 青海栽培何首乌中三种抗氧化活性芪多酚类物质的筛选、分离制备方法[P]. 2016-03-09.
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