VEGF(189) Expression Is Highly Related to Adaptation of the Plateau Pika (Ochotona curzoniae) Inhabiting High Altitudes

NWIPB OpenIR

	VEGF(189) Expression Is Highly Related to Adaptation of the Plateau Pika (Ochotona curzoniae) Inhabiting High Altitudes
	Li, Hongge 1,2; Guo, Songchang 1; Ren, Yongming 3; Wang, Depeng 1,2; Yu, Honghao 1,2; Li, Wenjing 1,2; Zhao, Xinquan 1; Chang, Zhijie 3; Zhao, XQ (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China.
	2013-12-01
发表期刊	HIGH ALTITUDE MEDICINE & BIOLOGY
ISSN	1527-0297
卷号	14 期号:4 页码:395-404
文章类型	Article
摘要	Li, Hongge, Songchang Guo, Yongming Ren, Depeng Wang, Honghao Yu, Wenjing Li, Xinquan Zhao, and Zhijie Chang. VEGF(189) expression is highly related to adaptation of the plateau pika (Ochotona curzoniae) inhabiting high altitudes. High Alt Med Biol 14:395-404, 2013. The plateau pika (Ochotona curzonia) has adapted to high-altitude hypoxia during evolution. Higher microvessel density in specific tissues and a blunted hypoxic pulmonary vasoconstriction response are the critical components of this adaptation. VEGF, vascular endothelial growth factor, has proved to be a key regulator of angiogenesis in response to tissue hypoxia and to play an important role in vascular vasodilation. However, the role of VEGF in adaptation to high-altitude hypoxia in the plateau pika remains unknown. In this study, we cloned cDNAs for VEGF(165) and VEGF(189) and examined their expression in pikas inhabiting altitudes of 3200 and 4750m. Phylogenetic analysis reveals that pika VEGF(165) and VEGF(189) are evolutionarily conserved. Real-time PCR analysis demonstrates that VEGF(165) and VEGF(189) display tissue and altitude-specific expression patterns. Interestingly, we found that the levels of VEGF(189) mRNA are significantly higher than those of VEGF(165) in the brain and muscle tissues of the pika, which is different from what was previously observed in sea-level mammals. VEGF(189) mRNA levels in brain, muscle, and lung of the pika increased with increased habitat altitude, whereas VEGF(165) shows less change. Our study suggests an important role for VEGF(189) in adaptation to hypoxia by the plateau pika in the high-altitude environment.; Li, Hongge, Songchang Guo, Yongming Ren, Depeng Wang, Honghao Yu, Wenjing Li, Xinquan Zhao, and Zhijie Chang. VEGF(189) expression is highly related to adaptation of the plateau pika (Ochotona curzoniae) inhabiting high altitudes. High Alt Med Biol 14:395-404, 2013. The plateau pika (Ochotona curzonia) has adapted to high-altitude hypoxia during evolution. Higher microvessel density in specific tissues and a blunted hypoxic pulmonary vasoconstriction response are the critical components of this adaptation. VEGF, vascular endothelial growth factor, has proved to be a key regulator of angiogenesis in response to tissue hypoxia and to play an important role in vascular vasodilation. However, the role of VEGF in adaptation to high-altitude hypoxia in the plateau pika remains unknown. In this study, we cloned cDNAs for VEGF(165) and VEGF(189) and examined their expression in pikas inhabiting altitudes of 3200 and 4750m. Phylogenetic analysis reveals that pika VEGF(165) and VEGF(189) are evolutionarily conserved. Real-time PCR analysis demonstrates that VEGF(165) and VEGF(189) display tissue and altitude-specific expression patterns. Interestingly, we found that the levels of VEGF(189) mRNA are significantly higher than those of VEGF(165) in the brain and muscle tissues of the pika, which is different from what was previously observed in sea-level mammals. VEGF(189) mRNA levels in brain, muscle, and lung of the pika increased with increased habitat altitude, whereas VEGF(165) shows less change. Our study suggests an important role for VEGF(189) in adaptation to hypoxia by the plateau pika in the high-altitude environment.
关键词	Adaptation Qinghai-tibetan Plateau Hypoxia Plateau Pika Vegf Real-time Pcr
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	ENDOTHELIAL GROWTH-FACTOR ; ZOKOR MYOSPALAX-BAILEYI ; FACTOR MESSENGER-RNA ; HYPOXIA-INDUCIBLE FACTOR-1-ALPHA ; DIFFERENTIAL EXPRESSION ; PULMONARY-CIRCULATION ; SYSTEMIC HYPOXIA ; VEGF ISOFORMS ; PROTEIN ; CELLS
收录类别	SCI
语种	英语
WOS研究方向	Biophysics ; Public, Environmental & Occupational Health ; Sport Sciences
WOS类目	Biophysics ; Public, Environmental & Occupational Health ; Sport Sciences
WOS记录号	WOS:000329162900015
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/3891
专题	中国科学院西北高原生物研究所
通讯作者	Zhao, XQ (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China.
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Tsinghua Univ, Sch Med, Dept Biol Sci & Biotechnol, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China
推荐引用方式 GB/T 7714	Li, Hongge,Guo, Songchang,Ren, Yongming,et al. VEGF(189) Expression Is Highly Related to Adaptation of the Plateau Pika (Ochotona curzoniae) Inhabiting High Altitudes[J]. HIGH ALTITUDE MEDICINE & BIOLOGY,2013,14(4):395-404.
APA	Li, Hongge.,Guo, Songchang.,Ren, Yongming.,Wang, Depeng.,Yu, Honghao.,...&Zhao, XQ .(2013).VEGF(189) Expression Is Highly Related to Adaptation of the Plateau Pika (Ochotona curzoniae) Inhabiting High Altitudes.HIGH ALTITUDE MEDICINE & BIOLOGY,14(4),395-404.
MLA	Li, Hongge,et al."VEGF(189) Expression Is Highly Related to Adaptation of the Plateau Pika (Ochotona curzoniae) Inhabiting High Altitudes".HIGH ALTITUDE MEDICINE & BIOLOGY 14.4(2013):395-404.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
VEGF189 Expression I（998KB）			开放获取	CC BY-NC-SA	浏览下载