Anti-fatigue activity of polysaccharides from the fruits of four Tibetan plateau indigenous medicinal plants

doi:10.1016/j.jep.2013.08.055

NWIPB OpenIR

	Anti-fatigue activity of polysaccharides from the fruits of four Tibetan plateau indigenous medicinal plants
	Ni, Weihua 1; Gao, Tingting 2; Wang, Hailiang 3; Du, Yuzhi 2; Li, Jiayu 4; Li, Cen 2; Wei, Lixin 2; Bi, Hongtao 2; Wei, LX (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Tibetan Med Pharmacol & Safety Ev, Xining 810008, Peoples R China.
	2013-11-25
发表期刊	JOURNAL OF ETHNOPHARMACOLOGY
ISSN	0378-8741
卷号	150 期号:2 页码:529-535
文章类型	Article
摘要	Ethnopharmacological relevance: The fruits of Hippophae rhamnoides L, Lycium barbarum L., Lycium ruthenicum Murr. and Nitraria tangutorum Bohr. are traditional medicinal food of Tibetans and used to alleviate fatigue caused by oxygen deficiency for thousands of years. The present study focused on exploiting natural polysaccharides with remarkable anti-fatigue activity from the four Qinghai-Tibet plateau characteristic berries. Materials and methods: The fruits of Hippophae rhamnoides, Lycium barbarum, Lycium ruthenicum and Nitraria tangutorum were collected from Haixi national municipality of Mongol and Tibetan (N 36.32 degrees, E98.11 degrees; altitude: 3100 m), Qinghai, China. Their polysaccharides (HRWP, LBWP, LRVVP and NTWP) were isolated by hot-water extraction, and purified by DEAE-Cellulose ion-exchange chromatography. The total carbohydrate, uronic acid, protein and starch contents of polysaccharides were determined by a spectrophotometric method. The molecular weight distributions of polysaccharides were determined by gel filtration chromatography. Their monosaccharide composition analysis was performed by the method of 1-phenyl-3-methyl-5-pyrazolone (PMP) pre-column derivatization and RP-HPLC analysis. HRWP, LBWP, LRWP and NTWP (50, 100 and 200 mg/kg) were orally administrated to mice once daily for 15 days, respectively. Anti-fatigue activity was assessed using the forced swim test (FST), and serum biochemical parameters were determined by an autoanalyzer and commercially available kits; the body and organs were also weighted. Result: LBWP, LRWP and NTWP were mainly composed of glucans and some RG-I pectins, and HRWP was mainly composed of HG-type pectin and some glucans. All the four polysaccharides decreased immobility in the EST, and the effects of LBWP and NTWP were demonstrated in lower doses compared with HRWP and LRWP. There was no significant difference in liver and heart indices between non-treated and polysaccharide-treated mice, but the spleen indices were increased in LBWP and NTWP (200 mg/kg) group. Moreover, the FST-induced reduction in glucose (Glc), superoxide dismutase (SOD) and glutathione peroxidase (GPx) and increase in creatine phosphokinase (CM), lactic dehydrogenase (LDH), blood urea nitrogen (BUN), triglyceride (TG) and malondialdehyde (MDA) levels, all indicators of fatigue, were inhibited by HRWP, LBWP, LRWP and NTWP to a certain extent while the effects of LBWP and NTWP were much better than that of HRWP and LRWP at the same dosage. Conclusion: Water-soluble polysaccharides HRWP, LBWP, LRWP and NTWP, from the fruits of four Tibetan plateau indigenous berry plants, significantly exhibited anti-fatigue activities for the first time, through triglyceride (TG) (or fat) mobilization during exercise and protecting corpuscular membrane by prevention of lipid oxidation via modifying several enzyme activities. Moreover, it is demonstrated that LBWP and NTWP are more potent than HRWP and LRWP, which were proposed to be applied in functional foods for anti-fatigue and antioxidant potential. Crown Copyright (C) 2013 Published by Elsevier Ireland Ltd. All rights reserved.; Ethnopharmacological relevance: The fruits of Hippophae rhamnoides L, Lycium barbarum L., Lycium ruthenicum Murr. and Nitraria tangutorum Bohr. are traditional medicinal food of Tibetans and used to alleviate fatigue caused by oxygen deficiency for thousands of years. The present study focused on exploiting natural polysaccharides with remarkable anti-fatigue activity from the four Qinghai-Tibet plateau characteristic berries.
关键词	Anti-fatigue Tibetan Plateau Characteristic Berry Polysaccharide Forced Swimming Test Anti-oxidant
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.jep.2013.08.055
关键词[WOS]	WATER-SOLUBLE POLYSACCHARIDES ; IMMUNOLOGICAL ACTIVITIES ; COLORIMETRIC METHOD ; MICE ; MITOCHONDRIA ; ANTIOXIDANT ; PERFORMANCE ; EXTRACTS ; CELLS ; MODEL
收录类别	SCI
语种	英语
项目资助者	Natural Science Foundation of Qinghai province(2011-Z-930Q) ; National Key Technology Research and Development Program of the Ministry of Science and Technology of China(2012BAI27B05)
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS类目	Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:000327567900014
引用统计	被引频次：133[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/3897
专题	中国科学院西北高原生物研究所
通讯作者	Wei, LX (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Tibetan Med Pharmacol & Safety Ev, Xining 810008, Peoples R China.
作者单位	1.Jilin Univ, Norman Bethune Coll Med, Dept Immunol, Changchun 130021, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Tibetan Med Pharmacol & Safety Ev, Xining 810008, Peoples R China 3.Jilin Univ, Hosp 2, Changchun 130033, Peoples R China 4.Jilin Univ, China Japan Union Hosp, Dept Cardiol, Changchun 130033, Peoples R China
推荐引用方式 GB/T 7714	Ni, Weihua,Gao, Tingting,Wang, Hailiang,et al. Anti-fatigue activity of polysaccharides from the fruits of four Tibetan plateau indigenous medicinal plants[J]. JOURNAL OF ETHNOPHARMACOLOGY,2013,150(2):529-535.
APA	Ni, Weihua.,Gao, Tingting.,Wang, Hailiang.,Du, Yuzhi.,Li, Jiayu.,...&Wei, LX .(2013).Anti-fatigue activity of polysaccharides from the fruits of four Tibetan plateau indigenous medicinal plants.JOURNAL OF ETHNOPHARMACOLOGY,150(2),529-535.
MLA	Ni, Weihua,et al."Anti-fatigue activity of polysaccharides from the fruits of four Tibetan plateau indigenous medicinal plants".JOURNAL OF ETHNOPHARMACOLOGY 150.2(2013):529-535.

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