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A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles
Qu, Fei ; Huang, Wei ; You, Jinmao
2018-02-01
发表期刊COLLOIDS AND SURFACES B-BIOINTERFACES
摘要In this work, we report a fluorescence strategy for detecting dopamine (DA) and sensing tyrosinase (TYR) activity on the basis of the dual-emission carbon dots (DECDs), which contain two emitters: the blue emitters (BE, maximum emission at 385 nm) and yellow emitters (YE, maximum emission at 530 nm). Gold nanoparticles (AuNPs) can effectively quench the two emissions of DECDs. The addition of DA aggregates AuNPs effectively, leading to the fluorescence recovery of dual emitters gradually. This strategy exhibits a high selectivity toward DA and shows good linear ranges, such as 0.5-3 mu M for BE and 0.1-3 mu M for YE. Additionally, the proposed method is successfully applied to the determination of DA in real samples with satisfactory recoveries. Subsequently, this DECDs-AuNPs platform is further taken advantage to assess TYR activity by the aid of TYR's capability for oxidation of DA into dopaquinone, which will not induce the agglomeration of AuNPs, so the fluorescence quenching of DECDs is associated with TYR activity. Finally, the mechanism of the reaction is discussed in detail, and the results suggest that both amine and phenolic hydroxyl groups of DA bring the aggregation of AuNPs. (C) 2017 Elsevier B.V. All rights reserved.
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/39102
专题中国科学院西北高原生物研究所
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GB/T 7714
Qu, Fei,Huang, Wei,You, Jinmao. A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2018.
APA Qu, Fei,Huang, Wei,&You, Jinmao.(2018).A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles.COLLOIDS AND SURFACES B-BIOINTERFACES.
MLA Qu, Fei,et al."A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles".COLLOIDS AND SURFACES B-BIOINTERFACES (2018).
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