NWIPB OpenIR
Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization
Ma, Tao ; Hu, Na ; Zhang, Qiulong ; Ding, Chenxi ; Che, Guodong ; Wu, Hongliang ; Sun, Yanan ; Ji, Zhongyin ; You, Jinmao ; Suo, Yourui ; Ding, Chenxu
2018
Source PublicationJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
AbstractA rapid and sensitive reversed-phase high performance liquid chromatography- fluorescence-mass spectrometer (RP-HPLC-FLD-MS) method based on pre-column derivatization using 2-(5-benzoacridine)ethyl-p-toluenesulfonate (BAETS) as labelling reagent has been developed for simultaneous determination of five triterpenic acids (asiatic acid (AA), maslinic acid (MA), corosolic acid (CA), oleanolic acid (OA), and betulinic acid (BA). The presented method was validated for linearity (correlation coefficient R-2>0.9994), precision (RSD 2.1%-3.9%) and reproducibility (RSD 0.01%-2.1%). The limits of detection (LODs) and the limits of quantitation LOQs were within the range of 0.71-1.02 ng mL(-1) and 2.28-3.24 ng mL(-1), respectively. The proposed method was successfully applied to simultaneously determine five triterpenic acids of four Corydalis plants and showed satisfactory reproducibility and credibility. Moreover, several main parameters affecting extraction procedure and derivatization efficiency were investigated by response surface methodology (RSM), respectively. Triterpenic acid content in four Corydalis plants was measured according to the established method and the results indicated that triterpenic acid contents were various in different organs and herbs.
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/39104
Collection中国科学院西北高原生物研究所
Recommended Citation
GB/T 7714
Ma, Tao,Hu, Na,Zhang, Qiulong,et al. Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2018.
APA Ma, Tao.,Hu, Na.,Zhang, Qiulong.,Ding, Chenxi.,Che, Guodong.,...&Ding, Chenxu.(2018).Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES.
MLA Ma, Tao,et al."Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES (2018).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ma, Tao]'s Articles
[Hu, Na]'s Articles
[Zhang, Qiulong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ma, Tao]'s Articles
[Hu, Na]'s Articles
[Zhang, Qiulong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ma, Tao]'s Articles
[Hu, Na]'s Articles
[Zhang, Qiulong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.