Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Preparative separation and purification of four cis-trans isomers of coumaroylspermidine analogs from safflower by high-speed counter-current chromatography | |
| Li, Wen-Cong1,2; Wang, Xiao-Yan2; Lin, Peng-Cheng3; Hu, Na1,2; Zhang, Qiu-Long1,2; Suo, You-Rui1; Ding, Chen-Xu1,3,4; Ding, CX (reprint author), Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining 810001, Peoples R China. | |
| 2013-11-01 | |
| 发表期刊 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| ISSN | 1570-0232 |
| 卷号 | 938页码:75-79 |
| 文章类型 | Article |
| 摘要 | High-speed counter-current chromatography (HSCCC) was successfully applied for the first time to isolate and purify four cis-trans isomers of coumaroylspermidine analogs from Safflower. HSCCC separation was achieved with a two-phase solvent system composed of chloroform-methanol-water (1:1:1, v/v/v) with the upper phase as the mobile phase. In a single run, a total of 1.3 mg of N-1, N-5, N-10-(E)-tri-p-coumaroylspermidine (EEE), 4.4 mg of N-1(E)-N-5-(Z)-N-10-(E)-tri-p-coumaroylspermidine (EZE), 7.2 mg of N-1(Z)-N-5-(z)-N-10-(E)-tri-p-coumaroylspermidine (ZZE), and 11.5 mg of N-1,N-5,N-10-(Z)-tri-p-coumaroylspermidine (ZZZ) were obtained from 100 mg of crude sample. High Performance Liquid Chromatography (HPLC) analysis showed that the purities of these four components are 95.5%, 98.1%, 97.5% and 96.2%, respectively. The chemical structures were identified by ESI-MS, H-1 NMR and C-13 NMR. (c) 2013 Elsevier B.V. All rights reserved.; High-speed counter-current chromatography (HSCCC) was successfully applied for the first time to isolate and purify four cis-trans isomers of coumaroylspermidine analogs from Safflower. HSCCC separation was achieved with a two-phase solvent system composed of chloroform-methanol-water (1:1:1, v/v/v) with the upper phase as the mobile phase. In a single run, a total of 1.3 mg of N-1, N-5, N-10-(E)-tri-p-coumaroylspermidine (EEE), 4.4 mg of N-1(E)-N-5-(Z)-N-10-(E)-tri-p-coumaroylspermidine (EZE), 7.2 mg of N-1(Z)-N-5-(z)-N-10-(E)-tri-p-coumaroylspermidine (ZZE), and 11.5 mg of N-1,N-5,N-10-(Z)-tri-p-coumaroylspermidine (ZZZ) were obtained from 100 mg of crude sample. High Performance Liquid Chromatography (HPLC) analysis showed that the purities of these four components are 95.5%, 98.1%, 97.5% and 96.2%, respectively. The chemical structures were identified by ESI-MS, H-1 NMR and C-13 NMR. (c) 2013 Elsevier B.V. All rights reserved. |
| 关键词 | Safflower Tri-p-coumaroylspermidine Hsccc |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1016/j.jchromb.2013.08.012 |
| 关键词[WOS] | CARTHAMUS-TINCTORIUS L. ; SEROTONIN TRANSPORTER INHIBITION ; TRI-P-COUMAROYLSPERMIDINE ; OIL CAKE ; DERIVATIVES ; N-(P-COUMAROYL)SEROTONIN ; FLORETS |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | Key Project of Chinese Ministry of Education(NO212188) ; Bureau of Science and Technology(2010-J-111) ; State Key Laboratory Breeding Base (Key Laboratory for Plateau Crop Germplasm Innovation and Utilization of Qinghai Province) |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000325734800011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/3911 |
| 专题 | 中国科学院西北高原生物研究所 |
| 通讯作者 | Ding, CX (reprint author), Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining 810001, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Qinghai Nationalities Univ, Xining 810007, Peoples R China 4.Key Lab Plateau Crop Germplasm Innovat & Utilizat, Xining 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Wen-Cong,Wang, Xiao-Yan,Lin, Peng-Cheng,et al. Preparative separation and purification of four cis-trans isomers of coumaroylspermidine analogs from safflower by high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2013,938:75-79. |
| APA | Li, Wen-Cong.,Wang, Xiao-Yan.,Lin, Peng-Cheng.,Hu, Na.,Zhang, Qiu-Long.,...&Ding, CX .(2013).Preparative separation and purification of four cis-trans isomers of coumaroylspermidine analogs from safflower by high-speed counter-current chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,938,75-79. |
| MLA | Li, Wen-Cong,et al."Preparative separation and purification of four cis-trans isomers of coumaroylspermidine analogs from safflower by high-speed counter-current chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 938(2013):75-79. |
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