Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Functional Characterization of the Poplar R2R3-MYB Transcription Factor PtoMYB216 Involved in the Regulation of Lignin Biosynthesis during Wood Formation | |
| Tian, Qiaoyan1; Wang, Xianqiang1; Li, Chaofeng1; Lu, Wanxiang1,2; Yang, Li1; Jiang, Yuanzhong1; Luo, Keming1,3; Luo, KM (reprint author), Southwest Univ, Chongqing Key Lab Transgen Plant & Safety Control, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Minist Educ,Inst Resources Bot,Sch Life Sci, Chongqing, Peoples R China. | |
| 2013-10-28 | |
| 发表期刊 | PLOS ONE
 |
| ISSN | 1932-6203 |
| 卷号 | 8期号:10 |
| 文章类型 | Article |
| 摘要 | Because of the importance of wood in many industrial applications, tremendous studies have been performed on wood formation, especially in lignin biosynthesis. MYB transcription factors (TFs), which consist of a large family of plant TFs, have been reported to directly regulate lignin biosynthetic genes in a number of plants. In this study, we describe the cloning and functional characterization of PtoMYB216, a cDNA isolated from Chinese white poplar (Populus tomentosa Carr.). PtoMYB216 encodes a protein belonging to the R2R3-MYB family and displays significant similarity with other MYB factors shown to regulate lignin synthesis in Arabidopsis. Gene expression profiling studies showed that PtoMYB216 mRNA is specifically expressed during secondary wall formation in wood. The 1.8-kb promoter sequence of PtoMYB216 was fused to the GUS coding sequence and introduced into wild-type A. thaliana. GUS expression was shown to be restricted to tissues undergoing secondary cell wall formation. Overexpression of PtoMYB216 specifically activated the expression of the upstream genes in the lignin biosynthetic pathway and resulted in ectopic deposition of lignin in cells that are normally unligninified. These results suggest that PtoMYB216 is specific transcriptional activators of lignin biosynthesis and involved in the regulation of wood formation in poplar.; Because of the importance of wood in many industrial applications, tremendous studies have been performed on wood formation, especially in lignin biosynthesis. MYB transcription factors (TFs), which consist of a large family of plant TFs, have been reported to directly regulate lignin biosynthetic genes in a number of plants. In this study, we describe the cloning and functional characterization of PtoMYB216, a cDNA isolated from Chinese white poplar (Populus tomentosa Carr.). PtoMYB216 encodes a protein belonging to the R2R3-MYB family and displays significant similarity with other MYB factors shown to regulate lignin synthesis in Arabidopsis. Gene expression profiling studies showed that PtoMYB216 mRNA is specifically expressed during secondary wall formation in wood. The 1.8-kb promoter sequence of PtoMYB216 was fused to the GUS coding sequence and introduced into wild-type A. thaliana. GUS expression was shown to be restricted to tissues undergoing secondary cell wall formation. Overexpression of PtoMYB216 specifically activated the expression of the upstream genes in the lignin biosynthetic pathway and resulted in ectopic deposition of lignin in cells that are normally unligninified. These results suggest that PtoMYB216 is specific transcriptional activators of lignin biosynthesis and involved in the regulation of wood formation in poplar. |
| WOS标题词 | Science & Technology |
| DOI | 10.1371/journal.pone.0076369 |
| 关键词[WOS] | CELL-WALL BIOSYNTHESIS ; ARABIDOPSIS-THALIANA ; PHENYLPROPANOID METABOLISM ; GENE-EXPRESSION ; DIRECT TARGET ; LIGNIFICATION ; POPULUS ; PATHWAY ; MYB ; DEPOSITION |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | National Natural Science Foundation of China(31171620) ; program for New Century Excellent Talents in University(NCET-11-0700) ; National Key Project for Research on Transgenic Plant(2011ZX08010-003) ; Research Fund for the Dectoral Program of Higher Education(20110182110004) ; 100 Talents Program of Chinese Academy of Sciences |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000326241200011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/3912 |
| 专题 | 中国科学院西北高原生物研究所 |
| 通讯作者 | Luo, KM (reprint author), Southwest Univ, Chongqing Key Lab Transgen Plant & Safety Control, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Minist Educ,Inst Resources Bot,Sch Life Sci, Chongqing, Peoples R China. |
| 作者单位 | 1.Southwest Univ, Chongqing Key Lab Transgen Plant & Safety Control, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Minist Educ,Inst Resources Bot,Sch Life Sci, Chongqing, Peoples R China 2.Southwest Univ, Coll Hort & Landscape Architecture, Chongqing, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Qiaoyan,Wang, Xianqiang,Li, Chaofeng,et al. Functional Characterization of the Poplar R2R3-MYB Transcription Factor PtoMYB216 Involved in the Regulation of Lignin Biosynthesis during Wood Formation[J]. PLOS ONE,2013,8(10). |
| APA | Tian, Qiaoyan.,Wang, Xianqiang.,Li, Chaofeng.,Lu, Wanxiang.,Yang, Li.,...&Luo, KM .(2013).Functional Characterization of the Poplar R2R3-MYB Transcription Factor PtoMYB216 Involved in the Regulation of Lignin Biosynthesis during Wood Formation.PLOS ONE,8(10). |
| MLA | Tian, Qiaoyan,et al."Functional Characterization of the Poplar R2R3-MYB Transcription Factor PtoMYB216 Involved in the Regulation of Lignin Biosynthesis during Wood Formation".PLOS ONE 8.10(2013). |
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