NWIPB OpenIR
miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis
Wang, Jian ; Tan, Jiaoyan ; Qi, Qi ; Yang, Lingzhi ; Wang, Yanhong ; Zhang, Chunlei ; Hu, Linyong ; Chen, Hong ; Fang, Xingtang
2018
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
AbstractMicroRNAs are endogenous, small non-coding RNAs that can play critical gene-regulatory roles during skeletal muscle development and are highly conserved. miR-487b-3p is expressed in muscle, and the detailed mechanism by which it regulates myoblast proliferation and differentiation has not been explored. Here, we found that miR-487b-3p expression was significantly higher in goat muscle tissues than in other tissues and was higher in fetal goat muscle tissues than in mature goat tissues, suggesting that miR-487b-3p has an important effect on skeletal muscle myogenesis. Functional studies showed that miR-487b-3p overexpression significantly suppressed C2C12 myoblast proliferation and differentiation, which was accompanied by the down-regulation of functional genes related to proliferation (MyoD, Pax7 and PCNA) and differentiation (Myf5, MyoG and Mef2c), whereas the inhibition of miR-487b-3p accelerated C2C12 myoblast proliferation and differentiation and was accompanied by the up-regulation of functional genes. Using Target-Scan and David, we found that miR-487b-3p targeted the 3'-UTR of IRS1, an essential regulator in the PI3K/Akt and MAPK/Erk pathways. We then confirmed the targeting of IRS1 by miR-487b-3p using dual-luciferase assays, RT-qPCR and western blotting. Furthermore, IRS1 silencing markedly inhibited proliferation and differentiation in cultured C2C12 myoblasts, confirming the important role of IRS1 in myogenesis. These results reveal an IRS1 -mediated regulatory link between miR-487b-3p and the PI3K/Akt and MAPK/Erk pathways during skeletal muscle myogenesis.
PublisherIVYSPRING INT PUBL
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/39317
Collection中国科学院西北高原生物研究所
Recommended Citation
GB/T 7714
Wang, Jian,Tan, Jiaoyan,Qi, Qi,et al. miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2018.
APA Wang, Jian.,Tan, Jiaoyan.,Qi, Qi.,Yang, Lingzhi.,Wang, Yanhong.,...&Fang, Xingtang.(2018).miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES.
MLA Wang, Jian,et al."miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES (2018).
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