Efficient purification of high-purity compounds from the stem of Lonicera japonica Thunb using two-dimensional preparative chromatography

doi:10.1002/jssc.201300319

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	Efficient purification of high-purity compounds from the stem of Lonicera japonica Thunb using two-dimensional preparative chromatography
	Jin, Hongli 1,2; Liu, Yanfang 1; Feng, Jiatao 1; Guo, Zhimou 1; Wang, Chaoran 1; Zhong, Zhengsheng 1; Peng, Xiaojun 2; Dang, Jun 3; Tao, Yanduo 3; Liang, Xinmiao 1; Liang, XM (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China.
	2013-08-01
发表期刊	JOURNAL OF SEPARATION SCIENCE
ISSN	1615-9306
卷号	36 期号:15 页码:2414-2420
文章类型	Article
摘要	Purification of high-purity compounds from traditional Chinese medicines (TCMs) plays an important role in investigating their bioactivity. Nevertheless, it is often quite difficult to isolate compounds with high purity because of the complexity of TCMs in chemical composition. In this work, a two-dimensional preparation method was successfully developed for the preparation of high-purity compounds from the stem of Lonicera japonica Thunb, based on two novel polar copolymerized RP stationary phases, XAqua C3 and XAqua C18. An XAqua C3 prep column was used to separate the sample in the first-dimensional preparation, and 14 g of sample was fractionated into eight fractions with a recovery of 82%. An XAqua C18 prep column was selected to prepare high-purity compounds in the second-dimensional preparation for its good orthogonality with the XAqua C3 stationary phase. As a result, major compounds in the sample were isolated with more than 99% purity. This method is a potent method to realize the efficient purification of compounds with high purity from the stem of L. japonica Thunb and it shows great potential in the separation of high-purity compounds from complex samples.; Purification of high-purity compounds from traditional Chinese medicines (TCMs) plays an important role in investigating their bioactivity. Nevertheless, it is often quite difficult to isolate compounds with high purity because of the complexity of TCMs in chemical composition. In this work, a two-dimensional preparation method was successfully developed for the preparation of high-purity compounds from the stem of Lonicera japonica Thunb, based on two novel polar copolymerized RP stationary phases, XAqua C3 and XAqua C18. An XAqua C3 prep column was used to separate the sample in the first-dimensional preparation, and 14 g of sample was fractionated into eight fractions with a recovery of 82%. An XAqua C18 prep column was selected to prepare high-purity compounds in the second-dimensional preparation for its good orthogonality with the XAqua C3 stationary phase. As a result, major compounds in the sample were isolated with more than 99% purity. This method is a potent method to realize the efficient purification of compounds with high purity from the stem of L. japonica Thunb and it shows great potential in the separation of high-purity compounds from complex samples.
关键词	High-purity Compound Lonicera Japonica
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1002/jssc.201300319
关键词[WOS]	PHASE LIQUID-CHROMATOGRAPHY ; STATIONARY PHASES ; IN-VITRO ; SEPARATION ; OPTIMIZATION ; PERFORMANCE ; COLUMN ; C18
收录类别	SCI
语种	英语
项目资助者	National Science and Technology Pillar Program(2008BAI51B01 ; 2012BAI29B08)
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000322709900005
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/3933
专题	中国科学院西北高原生物研究所
通讯作者	Liang, XM (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China.
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China 2.Dalian Univ Technol, Fac Chem Environm & Biol Sci & Technol, State Key Lab Fine Chem, Dalian, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Peoples R China
推荐引用方式 GB/T 7714	Jin, Hongli,Liu, Yanfang,Feng, Jiatao,et al. Efficient purification of high-purity compounds from the stem of Lonicera japonica Thunb using two-dimensional preparative chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2013,36(15):2414-2420.
APA	Jin, Hongli.,Liu, Yanfang.,Feng, Jiatao.,Guo, Zhimou.,Wang, Chaoran.,...&Liang, XM .(2013).Efficient purification of high-purity compounds from the stem of Lonicera japonica Thunb using two-dimensional preparative chromatography.JOURNAL OF SEPARATION SCIENCE,36(15),2414-2420.
MLA	Jin, Hongli,et al."Efficient purification of high-purity compounds from the stem of Lonicera japonica Thunb using two-dimensional preparative chromatography".JOURNAL OF SEPARATION SCIENCE 36.15(2013):2414-2420.

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