Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Cadmium interferes with maintenance of auxin homeostasis in Arabidopsis seedlings | |
Hu, Yan Feng1; Zhou, Guoying2; Na, Xiao Fan1; Yang, Lijing1; Nan, Wen Bin1; Liu, Xu1; Zhang, Yong Qiang1; Li, Jiao Long1; Bi, Yu Rong1; Bi, YR (reprint author), Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Cell Act & Stress Adaptat, Lanzhou 730000, Gansu, Peoples R China. | |
2013-07-15 | |
发表期刊 | JOURNAL OF PLANT PHYSIOLOGY |
ISSN | 0176-1617 |
卷号 | 170期号:11页码:965-975 |
文章类型 | Article |
摘要 | Auxin and its homeostasis play key roles in many aspects of plant growth and development. Cadmium (Cd) is a phytotoxic heavy metal and its inhibitory effects on plant growth and development have been extensively studied. However, the underlying molecular mechanism of the effects of Cd stress on auxin homeostasis is still unclear. In the present study, we found that the root elongation, shoot weight, hypocotyl length and chlorophyll content in wild-type (WT) Arabidopsis seedlings were significantly reduced after exposure to Cd stress. However, the lateral root (LR) formation was markedly promoted by Cd stress. The level and distribution of auxin were both greatly altered in primary root tips and cotyledons of Cd-treated plants. The results also showed that after Cd treatment, the IAA content was significantly decreased, which was accompanied by increases in the activity of the IAA oxidase and alteration in the expression of several putative auxin biosynthetic and catabolic genes. Application of the auxin transport inhibitor, 1-naphthylphthalamic acid (NPA) and 1-naphthoxyacetic acid (1-NOA), reversed the effects of Cd on LR formation. Additionally, there was less promotion of LR formation by Cd treatment in aux1-7 and pin2 mutants than that in the WT. Meanwhile, Cd stress also altered the expression of PINs and AUX1 in Arabidopsis roots, implying that the auxin transport pathway is required for Cd-modulated LR development. Taken together, these findings suggest that Cd stress disturbs auxin homeostasis through affecting auxin level, distribution, metabolism, and transport in Arabidopsis seedling. (C) 2013 Elsevier GmbH. All rights reserved.; Auxin and its homeostasis play key roles in many aspects of plant growth and development. Cadmium (Cd) is a phytotoxic heavy metal and its inhibitory effects on plant growth and development have been extensively studied. However, the underlying molecular mechanism of the effects of Cd stress on auxin homeostasis is still unclear. In the present study, we found that the root elongation, shoot weight, hypocotyl length and chlorophyll content in wild-type (WT) Arabidopsis seedlings were significantly reduced after exposure to Cd stress. However, the lateral root (LR) formation was markedly promoted by Cd stress. The level and distribution of auxin were both greatly altered in primary root tips and cotyledons of Cd-treated plants. The results also showed that after Cd treatment, the IAA content was significantly decreased, which was accompanied by increases in the activity of the IAA oxidase and alteration in the expression of several putative auxin biosynthetic and catabolic genes. Application of the auxin transport inhibitor, 1-naphthylphthalamic acid (NPA) and 1-naphthoxyacetic acid (1-NOA), reversed the effects of Cd on LR formation. Additionally, there was less promotion of LR formation by Cd treatment in aux1-7 and pin2 mutants than that in the WT. Meanwhile, Cd stress also altered the expression of PINs and AUX1 in Arabidopsis roots, implying that the auxin transport pathway is required for Cd-modulated LR development. Taken together, these findings suggest that Cd stress disturbs auxin homeostasis through affecting auxin level, distribution, metabolism, and transport in Arabidopsis seedling. (C) 2013 Elsevier GmbH. All rights reserved. |
关键词 | Arabidopsis Auxin Homeostasis Cadmium Heavy Metal Lateral Root |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
关键词[WOS] | BOX PROTEIN TIR1 ; MORPHOGENIC RESPONSES ; WATER-STRESS ; NITRIC-OXIDE ; PEA-PLANTS ; THALIANA ; COPPER ; BIOSYNTHESIS ; ACCUMULATION ; EXPRESSION |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Plant Sciences |
WOS类目 | Plant Sciences |
WOS记录号 | WOS:000322557100001 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/3934 |
专题 | 中国科学院西北高原生物研究所 |
通讯作者 | Bi, YR (reprint author), Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Cell Act & Stress Adaptat, Lanzhou 730000, Gansu, Peoples R China. |
作者单位 | 1.Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Cell Act & Stress Adaptat, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810008, Peoples R China |
推荐引用方式 GB/T 7714 | Hu, Yan Feng,Zhou, Guoying,Na, Xiao Fan,et al. Cadmium interferes with maintenance of auxin homeostasis in Arabidopsis seedlings[J]. JOURNAL OF PLANT PHYSIOLOGY,2013,170(11):965-975. |
APA | Hu, Yan Feng.,Zhou, Guoying.,Na, Xiao Fan.,Yang, Lijing.,Nan, Wen Bin.,...&Bi, YR .(2013).Cadmium interferes with maintenance of auxin homeostasis in Arabidopsis seedlings.JOURNAL OF PLANT PHYSIOLOGY,170(11),965-975. |
MLA | Hu, Yan Feng,et al."Cadmium interferes with maintenance of auxin homeostasis in Arabidopsis seedlings".JOURNAL OF PLANT PHYSIOLOGY 170.11(2013):965-975. |
条目包含的文件 | 下载所有文件 | |||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Cadmium interferes w(3113KB) | 开放获取 | CC BY-NC-SA | 浏览 下载 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论