Cloning and characterization of chromosomal markers in alfalfa (Medicago sativa L.)

doi:10.1007/s00122-013-2103-z

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	Cloning and characterization of chromosomal markers in alfalfa (Medicago sativa L.)
	Yu, Feng 1,2; Lei, Yunting 1; Li, Yuan 1,2; Dou, Quanwen 1; Wang, Haiqing 1; Chen, Zhiguo 1; Dou, QW (reprint author), Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Plateau Inst Biol, Xining 810001, Peoples R China.
	2013-07-01
发表期刊	THEORETICAL AND APPLIED GENETICS
ISSN	0040-5752
卷号	126 期号:7 页码:1885-1896
文章类型	Article
摘要	Eleven tandemly repetitive sequences were identified from a Cot-1 library by FISH and sequence analysis of alfalfa (Medicago sativa). Five repetitive sequences (MsCR-1, MsCR-2, MsCR-3, MsCR-4, and MsCR-5) were centromeric or pericentromeric, of which three were satellite DNAs and two were minisatellite DNAs. Monomers of 144, 148, and 168 bp were identified in MsCR-1, MsCR-2, and MsCR-3, respectively, while 15 and 39 bp monomers were identified in MsCR-4 and MsCR-5, respectively. Three repetitive sequences were characterized as subtelomeric; one repetitive sequence, MsTR-1, had a 184 bp monomer, and two repetitive sequences had fragments of 204 and 327 bp. Sequence analysis revealed homology (70-80 %) between MsTR-1 and a highly repeated sequence (C300) isolated from M. ssp. caerulea. Three identified repetitive sequences produced hybridization signals at multiple sites in a few of the chromosomes; one repetitive sequence was identified as the E180 satellite DNA previously isolated from M. sativa, while the other 163 and 227 bp fragments had distinct sequences. Physical mapping of the repetitive sequences with double-target FISH revealed different patterns. Thus, nine novel tandemly repetitive sequences that can be adopted as distinct chromosome markers in alfalfa were identified in this study. Furthermore, the chromosome distribution of each sequence was well described. Though significant chromosome variations were detected within and between cultivars, a molecular karyotype of alfalfa was suggested with the chromosome markers we identified. Therefore, these novel chromosome markers will still be a powerful tool for genome composition analysis, phylogenetic studies, and breeding applications.; Eleven tandemly repetitive sequences were identified from a Cot-1 library by FISH and sequence analysis of alfalfa (Medicago sativa). Five repetitive sequences (MsCR-1, MsCR-2, MsCR-3, MsCR-4, and MsCR-5) were centromeric or pericentromeric, of which three were satellite DNAs and two were minisatellite DNAs. Monomers of 144, 148, and 168 bp were identified in MsCR-1, MsCR-2, and MsCR-3, respectively, while 15 and 39 bp monomers were identified in MsCR-4 and MsCR-5, respectively. Three repetitive sequences were characterized as subtelomeric; one repetitive sequence, MsTR-1, had a 184 bp monomer, and two repetitive sequences had fragments of 204 and 327 bp. Sequence analysis revealed homology (70-80 %) between MsTR-1 and a highly repeated sequence (C300) isolated from M. ssp. caerulea. Three identified repetitive sequences produced hybridization signals at multiple sites in a few of the chromosomes; one repetitive sequence was identified as the E180 satellite DNA previously isolated from M. sativa, while the other 163 and 227 bp fragments had distinct sequences. Physical mapping of the repetitive sequences with double-target FISH revealed different patterns. Thus, nine novel tandemly repetitive sequences that can be adopted as distinct chromosome markers in alfalfa were identified in this study. Furthermore, the chromosome distribution of each sequence was well described. Though significant chromosome variations were detected within and between cultivars, a molecular karyotype of alfalfa was suggested with the chromosome markers we identified. Therefore, these novel chromosome markers will still be a powerful tool for genome composition analysis, phylogenetic studies, and breeding applications.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s00122-013-2103-z
关键词[WOS]	REPETITIVE DNA-SEQUENCES ; IN-SITU HYBRIDIZATION ; BETA-VULGARIS ; KARYOTYPE ; GENOME ; IDENTIFICATION ; FISH ; ORGANIZATION ; CENTROMERES ; TRUNCATULA
收录类别	SCI
语种	英语
项目资助者	Chinese Academy of Sciences(KSCX2-EW-Q-23)
WOS研究方向	Agriculture ; Plant Sciences ; Genetics & Heredity
WOS类目	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture
WOS记录号	WOS:000320866200017
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/3937
专题	中国科学院西北高原生物研究所
通讯作者	Dou, QW (reprint author), Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Plateau Inst Biol, Xining 810001, Peoples R China.
作者单位	1.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Plateau Inst Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yu, Feng,Lei, Yunting,Li, Yuan,et al. Cloning and characterization of chromosomal markers in alfalfa (Medicago sativa L.)[J]. THEORETICAL AND APPLIED GENETICS,2013,126(7):1885-1896.
APA	Yu, Feng.,Lei, Yunting.,Li, Yuan.,Dou, Quanwen.,Wang, Haiqing.,...&Dou, QW .(2013).Cloning and characterization of chromosomal markers in alfalfa (Medicago sativa L.).THEORETICAL AND APPLIED GENETICS,126(7),1885-1896.
MLA	Yu, Feng,et al."Cloning and characterization of chromosomal markers in alfalfa (Medicago sativa L.)".THEORETICAL AND APPLIED GENETICS 126.7(2013):1885-1896.

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