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Isolation and Characterization of cDNAs Encoding Leucoanthocyanidin Reductase and Anthocyanidin Reductase from Populus trichocarpa
Wang, Lijun1,2; Jiang, Yuanzhong1; Yuan, Li1; Lu, Wanxiang2,3; Yang, Li1; Karim, Abdul1; Luo, Keming1,2,3; Luo, KM (reprint author), Southwest Univ, Sch Life Sci, Inst Resources Bot, Minist Educ,Key Lab Ecoenvironm Three Gorges Rese, Chongqing, Peoples R China.
2013-05-31
发表期刊PLOS ONE
ISSN1932-6203
卷号8期号:5
文章类型Article
摘要Proanthocyanidins (PAs) contribute to poplar defense mechanisms against biotic and abiotic stresses. Transcripts of PA biosynthetic genes accumulated rapidly in response to infection by the fungus Marssonina brunnea f.sp. multigermtubi, treatments of salicylic acid (SA) and wounding, resulting in PA accumulation in poplar leaves. Anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) are two key enzymes of the PA biosynthesis that produce the main subunits: (+)-catechin and (-)-epicatechin required for formation of PA polymers. In Populus, ANR and LAR are encoded by at least two and three highly related genes, respectively. In this study, we isolated and functionally characterized genes PtrANR1 and PtrLAR1 from P. trichocarpa. Phylogenetic analysis shows that Populus ANR1 and LAR1 occurr in two distinct phylogenetic lineages, but both genes have little difference in their tissue distribution, preferentially expressed in roots. Overexpression of PtrANR1 in poplar resulted in a significant increase in PA levels but no impact on catechin levels. Antisense down-regulation of PtrANR1 showed reduced PA accumulation in transgenic lines, but increased levels of anthocyanin content. Ectopic expression of PtrLAR1 in poplar positively regulated the biosynthesis of PAs, whereas the accumulation of anthocyanin and flavonol was significantly reduced (P<0.05) in all transgenic plants compared to the control plants. These results suggest that both PtrANR1 and PtrLAR1 contribute to PA biosynthesis in Populus.; Proanthocyanidins (PAs) contribute to poplar defense mechanisms against biotic and abiotic stresses. Transcripts of PA biosynthetic genes accumulated rapidly in response to infection by the fungus Marssonina brunnea f.sp. multigermtubi, treatments of salicylic acid (SA) and wounding, resulting in PA accumulation in poplar leaves. Anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) are two key enzymes of the PA biosynthesis that produce the main subunits: (+)-catechin and (-)-epicatechin required for formation of PA polymers. In Populus, ANR and LAR are encoded by at least two and three highly related genes, respectively. In this study, we isolated and functionally characterized genes PtrANR1 and PtrLAR1 from P. trichocarpa. Phylogenetic analysis shows that Populus ANR1 and LAR1 occurr in two distinct phylogenetic lineages, but both genes have little difference in their tissue distribution, preferentially expressed in roots. Overexpression of PtrANR1 in poplar resulted in a significant increase in PA levels but no impact on catechin levels. Antisense down-regulation of PtrANR1 showed reduced PA accumulation in transgenic lines, but increased levels of anthocyanin content. Ectopic expression of PtrLAR1 in poplar positively regulated the biosynthesis of PAs, whereas the accumulation of anthocyanin and flavonol was significantly reduced (P<0.05) in all transgenic plants compared to the control plants. These results suggest that both PtrANR1 and PtrLAR1 contribute to PA biosynthesis in Populus.
WOS标题词Science & Technology
DOI10.1371/journal.pone.0064664
关键词[WOS]PROANTHOCYANIDIN SYNTHESIS ; FLAVONOID BIOSYNTHESIS ; TRANSCRIPTION FACTOR ; LOTUS-CORNICULATUS ; MOLECULAR-CLONING ; CONDENSED TANNINS ; EXPRESSION ; GENE ; BIOCHEMISTRY ; PATHWAYS
收录类别SCI
语种英语
项目资助者National Natural Science Foundation of China(31171620) ; program for New Century Excellent Talents in University(NCET-11-0700) ; Research Fund for the Doctoral Program of Higher Education(20110182110004)
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000319799900075
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/3939
专题中国科学院西北高原生物研究所
通讯作者Luo, KM (reprint author), Southwest Univ, Sch Life Sci, Inst Resources Bot, Minist Educ,Key Lab Ecoenvironm Three Gorges Rese, Chongqing, Peoples R China.
作者单位1.Southwest Univ, Sch Life Sci, Inst Resources Bot, Minist Educ,Key Lab Ecoenvironm Three Gorges Rese, Chongqing, Peoples R China
2.Southwest Univ, Coll Hort & Landscape Architecture, Chongqing, Peoples R China
3.Chinese Acad Sci, NorthWest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Peoples R China
推荐引用方式
GB/T 7714
Wang, Lijun,Jiang, Yuanzhong,Yuan, Li,et al. Isolation and Characterization of cDNAs Encoding Leucoanthocyanidin Reductase and Anthocyanidin Reductase from Populus trichocarpa[J]. PLOS ONE,2013,8(5).
APA Wang, Lijun.,Jiang, Yuanzhong.,Yuan, Li.,Lu, Wanxiang.,Yang, Li.,...&Luo, KM .(2013).Isolation and Characterization of cDNAs Encoding Leucoanthocyanidin Reductase and Anthocyanidin Reductase from Populus trichocarpa.PLOS ONE,8(5).
MLA Wang, Lijun,et al."Isolation and Characterization of cDNAs Encoding Leucoanthocyanidin Reductase and Anthocyanidin Reductase from Populus trichocarpa".PLOS ONE 8.5(2013).
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