Structures of (1 -> 6)-beta-D-glucans from Bulgaria inquinans (Fries) and their immunological activities

doi:10.1016/j.carbpol.2012.12.036

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	Structures of (1 -> 6)-beta-D-glucans from Bulgaria inquinans (Fries) and their immunological activities
	Bi, Hongtao 1,2; Gao, Tingting 1,2; Liu, Dongbo 1; Tai, Guihua 1; Wei, Min 1; Zhou, Yifa 1; Wei, M (reprint author), NE Normal Univ, Sch Life Sci, Changchun 130022, Peoples R China.
	2013-04-02
发表期刊	CARBOHYDRATE POLYMERS
ISSN	0144-8617
卷号	93 期号:2 页码:547-552
文章类型	Article
摘要	In previous study, an unbranched (1 -> 6)-beta-D-glucan with Mw 2.6 kDa was isolated from fruit bodies of Bulgaria inquinans (Fries). In present paper, three branched (1 -> 6)-beta-D-glucans were obtained from the water-extracted residues by a sequential KOH-extraction, namely BIK2, BIK10 and BIK30. Their molecular weights were determined to be 37.5 kDa (BIK2), 288.9 kDa (BIK10) and 175.5 kDa (BIK30). Structural analysis indicated that their backbones were substituted by single glucosyls at C-3 positions, the branching ratios were 0.01 (BIK2), 0.17 (BIK10), 0.25 (BIK30). Immunological tests showed that all the four beta-D-glucans could significantly increase the ConA or LPS-induced lymphocytes proliferation in vivo. Moreover, branched (1 -> 6)-beta-D-glucans have more significantly lymphocytes proliferation activities than unbranched (1 -> 6)-beta-D-glucan, and the effect of (1 -> 6)-beta-D-glucans on lymphocytes proliferation increases along with molecular weights. The present results well enrich the structure-activity relationships of (1 -> 6)-beta-D-glucan, and indicate (1 -> 6)-beta-D-glucans from B. inquinans (Fries) are potential immunostimulating agents. (c) 2012 Elsevier Ltd. All rights reserved.; In previous study, an unbranched (1 -> 6)-beta-D-glucan with Mw 2.6 kDa was isolated from fruit bodies of Bulgaria inquinans (Fries). In present paper, three branched (1 -> 6)-beta-D-glucans were obtained from the water-extracted residues by a sequential KOH-extraction, namely BIK2, BIK10 and BIK30. Their molecular weights were determined to be 37.5 kDa (BIK2), 288.9 kDa (BIK10) and 175.5 kDa (BIK30). Structural analysis indicated that their backbones were substituted by single glucosyls at C-3 positions, the branching ratios were 0.01 (BIK2), 0.17 (BIK10), 0.25 (BIK30). Immunological tests showed that all the four beta-D-glucans could significantly increase the ConA or LPS-induced lymphocytes proliferation in vivo. Moreover, branched (1 -> 6)-beta-D-glucans have more significantly lymphocytes proliferation activities than unbranched (1 -> 6)-beta-D-glucan, and the effect of (1 -> 6)-beta-D-glucans on lymphocytes proliferation increases along with molecular weights. The present results well enrich the structure-activity relationships of (1 -> 6)-beta-D-glucan, and indicate (1 -> 6)-beta-D-glucans from B. inquinans (Fries) are potential immunostimulating agents. (c) 2012 Elsevier Ltd. All rights reserved.
关键词	Bulgaria Inquinans (Fries) Polysaccharide (1 -> 6)-beta-d-glucan Lymphocyte Proliferation
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1016/j.carbpol.2012.12.036
关键词[WOS]	BETA-GLUCAN RECEPTOR ; EDIBLE MUSHROOM ; POLYSACCHARIDES ; RECOGNITION ; DECTIN-1 ; EXTRACT ; BODIES
收录类别	SCI
语种	英语
项目资助者	Chinese New Drug Creation and Manufacturing Program(2012ZX09502001-001) ; National Natural Science Foundation of China(31170770 ; 30973857)
WOS研究方向	Chemistry ; Polymer Science
WOS类目	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS记录号	WOS:000317148200026
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/3950
专题	中国科学院西北高原生物研究所
通讯作者	Wei, M (reprint author), NE Normal Univ, Sch Life Sci, Changchun 130022, Peoples R China.
作者单位	1.NE Normal Univ, Sch Life Sci, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Peoples R China
推荐引用方式 GB/T 7714	Bi, Hongtao,Gao, Tingting,Liu, Dongbo,et al. Structures of (1 -> 6)-beta-D-glucans from Bulgaria inquinans (Fries) and their immunological activities[J]. CARBOHYDRATE POLYMERS,2013,93(2):547-552.
APA	Bi, Hongtao.,Gao, Tingting.,Liu, Dongbo.,Tai, Guihua.,Wei, Min.,...&Wei, M .(2013).Structures of (1 -> 6)-beta-D-glucans from Bulgaria inquinans (Fries) and their immunological activities.CARBOHYDRATE POLYMERS,93(2),547-552.
MLA	Bi, Hongtao,et al."Structures of (1 -> 6)-beta-D-glucans from Bulgaria inquinans (Fries) and their immunological activities".CARBOHYDRATE POLYMERS 93.2(2013):547-552.

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