Molecular characterization of two y-type high molecular weight glutenin subunit alleles 1Ay12* and 1Ay8* from cultivated einkorn wheat (Triticum monococcum ssp monococcum)

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	*Molecular characterization of two y-type high molecular weight glutenin subunit alleles 1Ay12 and 1Ay8* from cultivated einkorn wheat (Triticum monococcum ssp monococcum)**
	Guo, Xiao-Hui 1,2; Wu, Bi-Hua 1,2; Hu, Xi-Gui 1,2; Bi, Zhe-Guang 1,2; Wang, Zhen-Zhen 1,2; Liu, Deng-Cai 1,2,3; Zheng, You-Liang 1,2; Wu, BH (reprint author), Sichuan Agr Univ, Triticeae Res Inst, Wenjiang 611130, Peoples R China.
	2013-03-01
发表期刊	GENE
ISSN	0378-1119
卷号	516 期号:1 页码:1-7
文章类型	Article
摘要	Two y-type high molecular weight glutenin subunits (HMW-GSs) 1Ay12* and 1Ay8* from the two accessions PI560720 and PI345186 of cultivated einkorn wheat (Triticum monococcum ssp. monococcum, M, 2n = 2x = 14), were identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The mobility of 1Ay12* and 1Ay8* was similar to that of 1Dy12 and 1By8 from common wheat Chinese Spring, respectively. Their ORFs respectively consisted of 1812 bp and 1935 bp, encoding 602 and 643 amino acid residues with the four typical structural domains of HMW-GS including signal peptide, conserved N-, and C-terminal and central repetitive domains. Compared with the most similar active 1Ay alleles previous published, there were a total of 15 SNPs and 2 InDels in them. Their encoding functions were confirmed by successful heterogeneous expression. The two novel 1Ay alleles were named as 1Ay12* and 1Ay8* with the accession No. JQ318694 and JQ318695 in GenBank, respectively. The two alleles were classed into the two distinct groups, Phe-type and Cys-type, which might be relevant to the differentiation of Glu-A1-2 alleles. Of which, 1Ay8* belonged to Cys-type group, and its protein possessed an additional conserved cysteine residue in central repetitive region besides the six common ones in N- and C-terminal regions of Phe-type group, and was the second longest in all the known active 1Ay alleles. These results suggested that the subunit 1Ay8* of cultivated einkom wheat accession PI345186 might have a potential ability to strengthen the gluten polymer interactions and be a valuable genetic resource for wheat quality improvement. (C) 2012 Elsevier B.V. All rights reserved.; Two y-type high molecular weight glutenin subunits (HMW-GSs) 1Ay12* and 1Ay8* from the two accessions PI560720 and PI345186 of cultivated einkorn wheat (Triticum monococcum ssp. monococcum, M, 2n = 2x = 14), were identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The mobility of 1Ay12* and 1Ay8* was similar to that of 1Dy12 and 1By8 from common wheat Chinese Spring, respectively. Their ORFs respectively consisted of 1812 bp and 1935 bp, encoding 602 and 643 amino acid residues with the four typical structural domains of HMW-GS including signal peptide, conserved N-, and C-terminal and central repetitive domains. Compared with the most similar active 1Ay alleles previous published, there were a total of 15 SNPs and 2 InDels in them. Their encoding functions were confirmed by successful heterogeneous expression. The two novel 1Ay alleles were named as 1Ay12* and 1Ay8* with the accession No. JQ318694 and JQ318695 in GenBank, respectively. The two alleles were classed into the two distinct groups, Phe-type and Cys-type, which might be relevant to the differentiation of Glu-A1-2 alleles. Of which, 1Ay8* belonged to Cys-type group, and its protein possessed an additional conserved cysteine residue in central repetitive region besides the six common ones in N- and C-terminal regions of Phe-type group, and was the second longest in all the known active 1Ay alleles. These results suggested that the subunit 1Ay8* of cultivated einkom wheat accession PI345186 might have a potential ability to strengthen the gluten polymer interactions and be a valuable genetic resource for wheat quality improvement. (C) 2012 Elsevier B.V. All rights reserved.
关键词	Triticum Monococcum Ssp Monococcum Hmw-gs 1ay Gene Heterogeneous Expression Differentiation
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	BREAD-MAKING QUALITY ; SEED STORAGE PROTEINS ; DURUM-WHEAT ; A-GENOME ; GENES ; GENETICS ; SEQUENCE ; URARTU ; WILD ; ELECTROPHORESIS
收录类别	SCI
语种	英语
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000315367500001
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/3956
专题	中国科学院西北高原生物研究所
通讯作者	Wu, BH (reprint author), Sichuan Agr Univ, Triticeae Res Inst, Wenjiang 611130, Peoples R China.
作者单位	1.Sichuan Agr Univ, Triticeae Res Inst, Wenjiang 611130, Peoples R China 2.Sichuan Agr Univ, Minist Educ, Key Lab Crop Genet Resources & Improvement, Yaan 625014, Peoples R China 3.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Guo, Xiao-Hui,Wu, Bi-Hua,Hu, Xi-Gui,et al. Molecular characterization of two y-type high molecular weight glutenin subunit alleles 1Ay12* and 1Ay8* from cultivated einkorn wheat (Triticum monococcum ssp monococcum)[J]. GENE,2013,516(1):1-7.
APA	Guo, Xiao-Hui.,Wu, Bi-Hua.,Hu, Xi-Gui.,Bi, Zhe-Guang.,Wang, Zhen-Zhen.,...&Wu, BH .(2013).Molecular characterization of two y-type high molecular weight glutenin subunit alleles 1Ay12* and 1Ay8* from cultivated einkorn wheat (Triticum monococcum ssp monococcum).GENE,516(1),1-7.
MLA	Guo, Xiao-Hui,et al."Molecular characterization of two y-type high molecular weight glutenin subunit alleles 1Ay12* and 1Ay8* from cultivated einkorn wheat (Triticum monococcum ssp monococcum)".GENE 516.1(2013):1-7.

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