Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Culturable actinomycetes from desert ecosystem in northeast of Qinghai-Tibet Plateau | |
| Ding, Dong1; Chen, Guichen1; Wang, Bochan2; Wang, Qilan1; Liu, Demei1; Peng, Min1; Shi, Ping2; Peng, M (reprint author), Chinese Acad Sci, NW Inst Plateau Biol, Xining 810008, Peoples R China. | |
| 2013-03-01 | |
| 发表期刊 | ANNALS OF MICROBIOLOGY
 |
| ISSN | 1590-4261 |
| 卷号 | 63期号:1页码:259-266 |
| 文章类型 | Article |
| 摘要 | In this study, 53 actinomycetes strains were isolated from desert ecosystems located in northeast of Qinghai-Tibet Plateau and grouped into four RFLP patterns. Twenty-six actinomycetes with obvious morphology differences were chosen for phylogenetic diversity study and antimicrobial testing. As a result, the 16S rRNA gene sequencing showed that these strains belonged to Streptomyces, Micromonospora, Saccharothrix, Streptosporangium and Cellulomonas, and that most of the strains had antimicrobial bioactivity. The PKS and NRPS genes detection indicated diversified potential bioactive products of actinomycetes in this ecosystem. Among these strains, Sd-31 was chosen to study the bioactive products using HPLC-MS because of its optimum antimicrobial bioactivity. The result showed that it might produce Granatomycin A, Granatomycin C, and an unknown compound.; In this study, 53 actinomycetes strains were isolated from desert ecosystems located in northeast of Qinghai-Tibet Plateau and grouped into four RFLP patterns. Twenty-six actinomycetes with obvious morphology differences were chosen for phylogenetic diversity study and antimicrobial testing. As a result, the 16S rRNA gene sequencing showed that these strains belonged to Streptomyces, Micromonospora, Saccharothrix, Streptosporangium and Cellulomonas, and that most of the strains had antimicrobial bioactivity. The PKS and NRPS genes detection indicated diversified potential bioactive products of actinomycetes in this ecosystem. Among these strains, Sd-31 was chosen to study the bioactive products using HPLC-MS because of its optimum antimicrobial bioactivity. The result showed that it might produce Granatomycin A, Granatomycin C, and an unknown compound. |
| 关键词 | Qinghai-tibet Plateau Actinomycete 16s Rrna-rflp Pks Nrps Desert Ecosystem |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 关键词[WOS] | 16S RIBOSOMAL-RNA ; SP NOV. ; STREPTOMYCES-LATERITIUS ; COMMUNITY COMPOSITION ; RAPID IDENTIFICATION ; SOIL ; DIVERSITY ; AMPLIFICATION ; PURIFICATION ; ANTIBIOTICS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS记录号 | WOS:000314692400028 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/3957 |
| 专题 | 中国科学院西北高原生物研究所 |
| 通讯作者 | Peng, M (reprint author), Chinese Acad Sci, NW Inst Plateau Biol, Xining 810008, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810008, Peoples R China 2.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Dong,Chen, Guichen,Wang, Bochan,et al. Culturable actinomycetes from desert ecosystem in northeast of Qinghai-Tibet Plateau[J]. ANNALS OF MICROBIOLOGY,2013,63(1):259-266. |
| APA | Ding, Dong.,Chen, Guichen.,Wang, Bochan.,Wang, Qilan.,Liu, Demei.,...&Peng, M .(2013).Culturable actinomycetes from desert ecosystem in northeast of Qinghai-Tibet Plateau.ANNALS OF MICROBIOLOGY,63(1),259-266. |
| MLA | Ding, Dong,et al."Culturable actinomycetes from desert ecosystem in northeast of Qinghai-Tibet Plateau".ANNALS OF MICROBIOLOGY 63.1(2013):259-266. |
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