NWIPB OpenIR
Responses of the functional structure of soil microbial community to livestock grazing in the Tibetan alpine grassland
Yang, Yunfeng1; Wu, Linwei1; Lin, Qiaoyan2,3; Yuan, Mengting1; Xu, Depeng1; Yu, Hao4,5,6,7; Hu, Yigang3,8; Duan, Jichuang3; Li, Xiangzhen9; He, Zhili4,5; Xue, Kai4,5; van Nostrand, Joy4,5; Wang, Shiping2,3; Zhou, Jizhong1,4,5,10; Yang, YF (reprint author), Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China.
2013-02-01
Source PublicationGLOBAL CHANGE BIOLOGY
ISSN1354-1013
Volume19Issue:2Pages:637-648
SubtypeArticle
AbstractMicrobes play key roles in various biogeochemical processes, including carbon (C) and nitrogen (N) cycling. However, changes of microbial community at the functional gene level by livestock grazing, which is a global land-use activity, remain unclear. Here we use a functional gene array, GeoChip 4.0, to examine the effects of free livestock grazing on the microbial community at an experimental site of Tibet, a region known to be very sensitive to anthropogenic perturbation and global warming. Our results showed that grazing changed microbial community functional structure, in addition to aboveground vegetation and soil geochemical properties. Further statistical tests showed that microbial community functional structures were closely correlated with environmental variables, and variations in microbial community functional structures were mainly controlled by aboveground vegetation, soil C/N ratio, and NH4+-N. In-depth examination of N cycling genes showed that abundances of N mineralization and nitrification genes were increased at grazed sites, but denitrification and N-reduction genes were decreased, suggesting that functional potentials of relevant bioprocesses were changed. Meanwhile, abundances of genes involved in methane cycling, C fixation, and degradation were decreased, which might be caused by vegetation removal and hence decrease in litter accumulation at grazed sites. In contrast, abundances of virulence, stress, and antibiotics resistance genes were increased because of the presence of livestock. In conclusion, these results indicated that soil microbial community functional structure was very sensitive to the impact of livestock grazing and revealed microbial functional potentials in regulating soil N and C cycling, supporting the necessity to include microbial components in evaluating the consequence of land-use and/or climate changes.; Microbes play key roles in various biogeochemical processes, including carbon (C) and nitrogen (N) cycling. However, changes of microbial community at the functional gene level by livestock grazing, which is a global land-use activity, remain unclear. Here we use a functional gene array, GeoChip 4.0, to examine the effects of free livestock grazing on the microbial community at an experimental site of Tibet, a region known to be very sensitive to anthropogenic perturbation and global warming. Our results showed that grazing changed microbial community functional structure, in addition to aboveground vegetation and soil geochemical properties. Further statistical tests showed that microbial community functional structures were closely correlated with environmental variables, and variations in microbial community functional structures were mainly controlled by aboveground vegetation, soil C/N ratio, and NH4+-N. In-depth examination of N cycling genes showed that abundances of N mineralization and nitrification genes were increased at grazed sites, but denitrification and N-reduction genes were decreased, suggesting that functional potentials of relevant bioprocesses were changed. Meanwhile, abundances of genes involved in methane cycling, C fixation, and degradation were decreased, which might be caused by vegetation removal and hence decrease in litter accumulation at grazed sites. In contrast, abundances of virulence, stress, and antibiotics resistance genes were increased because of the presence of livestock. In conclusion, these results indicated that soil microbial community functional structure was very sensitive to the impact of livestock grazing and revealed microbial functional potentials in regulating soil N and C cycling, supporting the necessity to include microbial components in evaluating the consequence of land-use and/or climate changes.
KeywordClimate Change Gene Diversity Microbial Community Summer Grazing Tibetan Alpine Grassland
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1111/gcb.12065
WOS Keyword16S RIBOSOMAL-RNA ; SEA OIL PLUME ; NITROUS-OXIDE ; LAND-USE ; VEGETATION DYNAMICS ; ECOSYSTEM FUNCTION ; INNER-MONGOLIA ; DIVERSITY ; BIODIVERSITY ; PLATEAU
Indexed BySCI
Language英语
Funding OrganizationNational Science Foundation of China(41171201) ; State Key Joint Laboratory of Environment Simulation and Pollution Control(11Z03ESPCT) ; National Basic Research Program(2010CB833502) ; United States Department of Energy, Biological Systems Research on the Role of Microbial Communities in C Cycling Program(DE-SC0004601) ; Oklahoma Bioenergy Center (OBC) ; ENIGMA-Ecosystems ; Networks Integrated ; Genes and Molecular Assemblies through the Office of Science, Office of Biological and Environmental Research, of the U.S. Department of Energy(DE-AC02-05CH11231) ; United States Department of Agriculture through NSF-USDA Microbial Observatories Program(2007-35319-18305)
WOS Research AreaBiodiversity & Conservation ; Environmental Sciences & Ecology
WOS SubjectBiodiversity Conservation ; Ecology ; Environmental Sciences
WOS IDWOS:000314219200026
Citation statistics
Cited Times:85[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/3965
Collection中国科学院西北高原生物研究所
Corresponding AuthorYang, YF (reprint author), Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China.
Affiliation1.Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China
2.Chinese Acad Sci, Inst Tibetan Plateau Res, Lab Alpine Ecol & Biodivers, Beijing 100101, Peoples R China
3.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adapt & Evolut Plateau Biota, Xining 810008, Peoples R China
4.Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA
5.Univ Oklahoma, Dept Bot & Microbiol, Norman, OK 73019 USA
6.Harbin Inst Technol, State Key Lab Urban Water Resource & Environm, Harbin 150090, Peoples R China
7.Liaoning Tech Univ, Coll Resource & Environm Engn, Buxin 123000, Liaoning, Peoples R China
8.Chinese Acad Sci Lanzhou, Shapotou Desert Expt & Res Stn, Cold & Arid Reg & Environm & Engn Res Inst, Lanzhou 730000, Peoples R China
9.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China
10.Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, Berkeley, CA 94720 USA
Recommended Citation
GB/T 7714
Yang, Yunfeng,Wu, Linwei,Lin, Qiaoyan,et al. Responses of the functional structure of soil microbial community to livestock grazing in the Tibetan alpine grassland[J]. GLOBAL CHANGE BIOLOGY,2013,19(2):637-648.
APA Yang, Yunfeng.,Wu, Linwei.,Lin, Qiaoyan.,Yuan, Mengting.,Xu, Depeng.,...&Yang, YF .(2013).Responses of the functional structure of soil microbial community to livestock grazing in the Tibetan alpine grassland.GLOBAL CHANGE BIOLOGY,19(2),637-648.
MLA Yang, Yunfeng,et al."Responses of the functional structure of soil microbial community to livestock grazing in the Tibetan alpine grassland".GLOBAL CHANGE BIOLOGY 19.2(2013):637-648.
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