蕨麻中三萜类化合物的提取、分离纯化工艺研究

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	蕨麻中三萜类化合物的提取、分离纯化工艺研究
	王晓虹
学位类型	硕士
导师	韩发
	2014-05
学位授予单位	中国科学院研究生院
学位授予地点	北京
学位专业	生态学
关键词	蕨麻三萜提取分离
摘要	蕨麻学名为鹅绒委陵菜(Potentilla anserina L.)，又名人参果、延寿果等。属蔷薇科 (Rosacrae)，委陵菜属(Potentilla)，为多年生草本，匍匐茎克隆植物。主要分布在青藏高原及其周边地区，藏医学中常用蕨麻入药，是一种重要的药食两用植物资源，在青藏高原地区具有悠久的应用历史。本课题对蕨麻中三萜类化合物成分提取、分离及纯化的工艺条件进行探索研究，并对比蕨麻中总三萜含量的测定方法、大孔树脂分离纯化蕨麻总三萜等条件进行优化选择，为实现蕨麻中三萜类化合物的有效利用和产业开发提供技术依据，主要研究结果如下： 1、试验优化选择确定的最佳蕨麻总三萜测定条件为：以5%香草醛-冰醋酸溶液 0.2mL 为显色剂，高氯酸溶液取0.8mL 为稳定剂，在70℃水浴20min，冷却后加入冰醋酸5mL，混匀后室温放置20min，在波长547nm 处测定吸光度A 值，在此条件下可得到较为可靠、稳定的吸光度值，可作为蕨麻中总三萜含量的定量测定方法参考。 2、利用单因素试验及响应面优化选择得到蕨麻总三萜的提取工艺条件为：提取时间80min，提取温度61℃，液料比26mL/g，提取2 次，通过重复验证试验表明蕨麻总三萜提取得率的平均值为1.08%，RSD=1.9%，与响应面拟合模型预测的总三萜提取得率的理论值1.14%基本吻合，表明建立的数学模型具有良好的预测性，所选工艺条件重现性好。 3、AB-8 型大孔树脂对蕨麻三萜有较好的分离纯化性能，通过动态吸附性考察试验得到，较优吸附条件为:上样量15mL，样品溶液pH 为5，上样液流速为0.5mL/min；响应曲面法得到最优解吸工艺条件为：乙醇浓度90%，解吸液用量17mL，解吸液流速 1.70mL/min，此条件下，回收率达86.28%，蕨麻总三萜含量为1.87mg/mL。该工艺条件操作简便、可操作且效果较好，因此，对实际生产具有一定的指导意义。
其他摘要	Potentilla anserina L. is a kind of perennial herb and a typical clonal plant stolons. Its also known as ginseng fruit, fruit of life extension and so on. It belongs to genus Potentilla, family Rose.Potentilla anserina L. are mainly distributed throughout the Tibetan plateau and its surrounding areas,Its commonly used as a kind of Tibetan medicine. Therefor, Potentilla anserina L. is an important medicinal and edible plant resources. In the Tibetan plateau it has a long history of application. In this research, the components of triterpenoids from Potentilla anserina L. were studied. The main contents were extraction, separation and purification conditions of triterpenoids. Determination of total triterpenoids by colorimetry, optimize the separation and purification technology of macroporous resins to total triterpenoids. To achieve efficient exploration and use of triterpenoids from Potentilla anserina L., the main results and conclusions were obtained as follows: 1.Selected the optimum measurement conditions by 5% vanillin-acetic acid of total triterpenoids from Potentilla anserina L., the results were obtained as follows: 0.2mL 5% vanillin-acetic acid as chromogenic reagent, 0.8mL perchloric acid as stabilizer, water bath for 20min under 70℃, added 5mL glacial acetic acid until it cool , mixed them and put it for 20min under room temperature, and measured the value of absorbance at wavelength of 547nm. The absorbance values under this condition were reliable and stable that can be used as fern quantitative determination of total triterpenoid from Potentilla anserina L.. 2.The methods of single factor test and response surface methodology were adopted to optimize the extraction process of total triterpenoids from Potentilla anserina L..Three factors including extraction temperature,extraction times and the ratio of liquid to material were selected with the Box-Behnken design. Response surface methodology showed the optimal technological conditions of total triterpenoids extraction: extraction time 80min,extraction temperature 61℃, the ratio of liquid to material 26mL/g and twice for extraction. Under this conditions,the extracting yield of total triterpenoids was up to 1.08%, RSD=1.9%, which is well matched with the predictive yield 1.14%. 3.AB-8 macroporous resin had the better adsorption and desorption ability to Potentilla triterpenoids. The test of optimum conditions for adsorption showed the results as follows: 15mL, pH5, velocity of sample solution 0.5mL/min; Response surface methodology showed the optimal desorption conditions were: ethanol concentration of 90%, the amount of desorption solution 17mL, desorption flow rate 1.70mL/min. Under this condition, the recovery rate of triterpenoids were 86.28% , and total triterpene content of Potentilla 1.87mg/mL. Thus, the separation and purification process was convenient, feasible and instructive.
文献类型	学位论文
条目标识符	http://210.75.249.4/handle/363003/4019
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	王晓虹. 蕨麻中三萜类化合物的提取、分离纯化工艺研究[D]. 北京. 中国科学院研究生院,2014.

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