LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent

NWIPB OpenIR

	LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent
	Ding, Chenxu ; Wang, Lingyun ; Tian, Chunliang ; Li, Yulin ; Sun, Zhiwei ; Wang, Honglun ; Suo, Yourui ; You, Jinmao
	2008-12-01
发表期刊	CHROMATOGRAPHIA ; Ding Chenxu,Wang Lingyun,Tian Chunliang,Li Yulin,Sun Zhiwei,Wang Honglun,Suo Yourui,You Jinmao. LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent.CHROMATOGRAPHIA,2008,68(11-12):893-902
摘要	A novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) coupling to liquid chromatography with electrospray ionization mass spectrometry for the detection of carbohydrates from the derivatized rape bee pollen samples is reported. Carbohydrates are derivatized to their bis-NMP-labeled derivatives. Derivatives showed an intense protonated molecular ion at m/z [M+H](+) in positive-ion detection mode. The mass-to-charge ratios of characteristic fragment ions at m/z 473.0 could be used for the accurately qualitative analysis of carbohydrates. This characteristic fragment ion is from the cleavage of C2-C3 bond in carbohydrate chain giving the specific fragment ions at m/z [MH-CmH2m+1Om-H2O](+) for pentose, hexose and glyceraldehydes and at m/z [MH-CmH2m-1Om+1-H2O](+) for alduronic acids such as galacturonic acid and glucuronic acid (m = n - 2, n is carbon number of carbohydrate). No interferences for all aliphatic and aromatic aldehydes presented in natural environmental samples were observed due to the highly specific parent mass-to-charge ratio and the characteristic fragment ions. The method, in conjunction with a gradient elution, offered a baseline resolution of carbohydrate derivatives on a reversed-phase Hypersil ODS-2 column. The carbohydrates such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be detected.; A novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) coupling to liquid chromatography with electrospray ionization mass spectrometry for the detection of carbohydrates from the derivatized rape bee pollen samples is reported. Carbohydrates are derivatized to their bis-NMP-labeled derivatives. Derivatives showed an intense protonated molecular ion at m/z [M+H](+) in positive-ion detection mode. The mass-to-charge ratios of characteristic fragment ions at m/z 473.0 could be used for the accurately qualitative analysis of carbohydrates. This characteristic fragment ion is from the cleavage of C2-C3 bond in carbohydrate chain giving the specific fragment ions at m/z [MH-C(m)H(2m+1)O(m)-H(2)O](+) for pentose, hexose and glyceraldehydes and at m/z [MH-C(m)H(2m-1)O(m+1)-H(2)O](+) for alduronic acids such as galacturonic acid and glucuronic acid (m = n - 2, n is carbon number of carbohydrate). No interferences for all aliphatic and aromatic aldehydes presented in natural environmental samples were observed due to the highly specific parent mass-to-charge ratio and the characteristic fragment ions. The method, in conjunction with a gradient elution, offered a baseline resolution of carbohydrate derivatives on a reversed-phase Hypersil ODS-2 column. The carbohydrates such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be detected.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/40503
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Ding, Chenxu,Wang, Lingyun,Tian, Chunliang,et al. LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent[J]. CHROMATOGRAPHIA, Ding Chenxu,Wang Lingyun,Tian Chunliang,Li Yulin,Sun Zhiwei,Wang Honglun,Suo Yourui,You Jinmao. LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent.CHROMATOGRAPHIA,2008,68(11-12):893-902,2008.
APA	Ding, Chenxu.,Wang, Lingyun.,Tian, Chunliang.,Li, Yulin.,Sun, Zhiwei.,...&You, Jinmao.(2008).LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent.CHROMATOGRAPHIA.
MLA	Ding, Chenxu,et al."LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent".CHROMATOGRAPHIA (2008).