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Proteomic detection of changes in protein expression induced by cordycepin in human hepatocellular carcinoma BEL-7402 cells
Shi, Ping ; Huang, Zhiwei ; Tan, Xiaorong ; Chen, Guichen
2008-06-01
发表期刊METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY ; Shi Ping,Huang Zhiwei,Tan Xiaorong,Chen Guichen.Proteomic detection of changes in protein expression induced by cordycepin in human hepatocellular carcinoma BEL-7402 cells.METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY,2008,30(5):347-353
摘要The nucleoside analogue cordycepin (3'-deoxyodenosine, 3'-dA), one of the components of cordyceps militaris, has been shown to inhibit the growth of various tumor cells. However, the probable mechanism is still obscure. In this study, the inhibition of cell growth and changes in protein expression induced by cordycepin were investigated in BEL-7402 cells. Using the MTT assay and flow cytometry, we found that cordycepin inhibits cell viability and induces apoptosis in BEL 7402 cells. Additionally. the proteins were separated using two-dimensional polyacrylamide gel electrophoresis, and eight proteins were found to be significantly, affected by cordycepin compared to untreated control; among them, two were downregulated and six were upregulated. Of the eight proteins, six were identified with peptide mass fingerprinting using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) after in-gel trypsin digestion. These proteins are involved in various aspects of cellular metabolism. It is suggested that the effect of cordycepin on the growth of tumor cells is significantly related to the metabolism-associated protein expression induced by cordycepin. Copyright 2008 Prous Science, S.A.U. or its licensors. All rights reserved.; The nucleoside analogue cordycepin (3'-deoxyodenosine, 3'-dA), one of the components of cordyceps militaris, has been shown to inhibit the growth of various tumor cells. However, the probable mechanism is still obscure. In this study, the inhibition of cell growth and changes in protein expression induced by cordycepin were investigated in BEL-7402 cells. Using the MTT assay and flow cytometry, we found that cordycepin inhibits cell viability and induces apoptosis in BEL 7402 cells. Additionally. the proteins were separated using two-dimensional polyacrylamide gel electrophoresis, and eight proteins were found to be significantly, affected by cordycepin compared to untreated control; among them, two were downregulated and six were upregulated. Of the eight proteins, six were identified with peptide mass fingerprinting using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) after in-gel trypsin digestion. These proteins are involved in various aspects of cellular metabolism. It is suggested that the effect of cordycepin on the growth of tumor cells is significantly related to the metabolism-associated protein expression induced by cordycepin. Copyright 2008 Prous Science, S.A.U. or its licensors. All rights reserved.
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/40511
专题中国科学院西北高原生物研究所
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GB/T 7714
Shi, Ping,Huang, Zhiwei,Tan, Xiaorong,et al. Proteomic detection of changes in protein expression induced by cordycepin in human hepatocellular carcinoma BEL-7402 cells[J]. METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY, Shi Ping,Huang Zhiwei,Tan Xiaorong,Chen Guichen.Proteomic detection of changes in protein expression induced by cordycepin in human hepatocellular carcinoma BEL-7402 cells.METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY,2008,30(5):347-353,2008.
APA Shi, Ping,Huang, Zhiwei,Tan, Xiaorong,&Chen, Guichen.(2008).Proteomic detection of changes in protein expression induced by cordycepin in human hepatocellular carcinoma BEL-7402 cells.METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY.
MLA Shi, Ping,et al."Proteomic detection of changes in protein expression induced by cordycepin in human hepatocellular carcinoma BEL-7402 cells".METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY (2008).
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