Functional Evolution of Leptin of Ochotona curzoniae in Adaptive Thermogenesis Driven by Cold Environmental Stress

NWIPB OpenIR

	Functional Evolution of Leptin of Ochotona curzoniae in Adaptive Thermogenesis Driven by Cold Environmental Stress
	Yang, Jie ; Bromage, Timothy G. ; Zhao, Qian ; Xu, Bao Hong ; Gao, Wei Li ; Tian, Hui Fang ; Tang, Hui Jun ; Liu, Dian Wu ; Zhao, Xin Quan
	2011-06-16
发表期刊	PLOS ONE ; Yang, J; Bromage, TG; Zhao, Q; Xu, BH; Gao, WL; Tian, HF; Tang, HJ; Liu, DW; Zhao, XQ.Functional Evolution of Leptin of Ochotona curzoniae in Adaptive Thermogenesis Driven by Cold Environmental Stress,PLOS ONE,2011,6(6):
摘要	Background: Environmental stress can accelerate the directional selection and evolutionary rate of specific stress-response proteins to bring about new or altered functions, enhancing an organism's fitness to challenging environments. Plateau pika (Ochotona curzoniae), an endemic and keystone species on Qinghai-Tibetan Plateau, is a high hypoxia and low temperature tolerant mammal with high resting metabolic rate and non-shivering thermogenesis to cope in this harsh plateau environment. Leptin is a key hormone related to how these animals regulate energy homeostasis. Previous molecular evolutionary analysis helped to generate the hypothesis that adaptive evolution of plateau pika leptin may be driven by cold stress. Methodology/Principal Findings: To test the hypothesis, recombinant pika leptin was first purified. The thermogenic characteristics of C57BL/6J mice injected with pika leptin under warm (23+/-1 degrees C) and cold (5+/-1 degrees C) acclimation is investigated. Expression levels of genes regulating adaptive thermogenesis in brown adipose tissue and the hypothalamus are compared between pika leptin and human leptin treatment, suggesting that pika leptin has adaptively and functionally evolved. Our results show that pika leptin regulates energy homeostasis via reduced food intake and increased energy expenditure under both warm and cold conditions. Compared with human leptin, pika leptin demonstrates a superior induced capacity for adaptive thermogenesis, which is reflected in a more enhanced beta-oxidation, mitochondrial biogenesis and heat production. Moreover, leptin treatment combined with cold stimulation has a significant synergistic effect on adaptive thermogenesis, more so than is observed with a single cold exposure or single leptin treatment. Conclusions/Significance: These findings support the hypothesis that cold stress has driven the functional evolution of plateau pika leptin as an ecological adaptation to the Qinghai-Tibetan Plateau.; Background: Environmental stress can accelerate the directional selection and evolutionary rate of specific stress-response proteins to bring about new or altered functions, enhancing an organism's fitness to challenging environments. Plateau pika (Ochotona curzoniae), an endemic and keystone species on Qinghai-Tibetan Plateau, is a high hypoxia and low temperature tolerant mammal with high resting metabolic rate and non-shivering thermogenesis to cope in this harsh plateau environment. Leptin is a key hormone related to how these animals regulate energy homeostasis. Previous molecular evolutionary analysis helped to generate the hypothesis that adaptive evolution of plateau pika leptin may be driven by cold stress.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/40828
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yang, Jie,Bromage, Timothy G.,Zhao, Qian,et al. Functional Evolution of Leptin of Ochotona curzoniae in Adaptive Thermogenesis Driven by Cold Environmental Stress[J]. PLOS ONE, Yang, J; Bromage, TG; Zhao, Q; Xu, BH; Gao, WL; Tian, HF; Tang, HJ; Liu, DW; Zhao, XQ.Functional Evolution of Leptin of Ochotona curzoniae in Adaptive Thermogenesis Driven by Cold Environmental Stress,PLOS ONE,2011,6(6):,2011.
APA	Yang, Jie.,Bromage, Timothy G..,Zhao, Qian.,Xu, Bao Hong.,Gao, Wei Li.,...&Zhao, Xin Quan.(2011).Functional Evolution of Leptin of Ochotona curzoniae in Adaptive Thermogenesis Driven by Cold Environmental Stress.PLOS ONE.
MLA	Yang, Jie,et al."Functional Evolution of Leptin of Ochotona curzoniae in Adaptive Thermogenesis Driven by Cold Environmental Stress".PLOS ONE (2011).