Knowledge Management System of Northwest Institute of Plateau Biology, CAS
LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification | |
Kang, Li ; You, Jinmao ; Sun, Zhiwei ; Wang, Chengyan ; Ji, Zhongyin ; Gao, Yongping ; Suo, Yourui ; Li, Yulin | |
2011 | |
发表期刊 | CHROMATOGRAPHIA ; Kang, L; You, JM; Sun, ZW; Wang, CY; Ji, ZY; Gao, YP; Suo, YR; Li, YL.LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification,CHROMATOGRAPHIA,2011,73(1-2):43-50 |
摘要 | A pre-column derivatization method for the simple, sensitive determination of biogenic amines using 10-ethyl-acridine-3-sulfonyl chloride (EASC) as labeling reagent with fluorescence detection and mass spectrometry (MS) identification has been developed. After pre-column derivatization, the labeled biogenic amines were separated on a Hypersil BDS-C18 column by gradient elution. The derivatives showed an intense protonated molecular ion corresponding m/z [M + H](+) in positive-ion mode. The collision-induced dissociation of protonated molecular ion formed specific fragment ions at m/z 196.5, m/z 222.7, m/z 224.4 and m/z 272.5, m/z 286.2. Satisfactory linear responses were observed at the concentration range of 0.02-10 mu mol L(-1) with coefficients of > 0.9993. Detection limits obtained by the analysis of a derivatized standard containing 0.2 pmol of each biogenic amine, were from 20.22 to 109.2 fmol (at a signal-to-noise ratio of 3). The relative standard deviations of retention times and peak areas for each biogenic amine were < 0.96 and 3.22%, respectively. Recoveries except for PUT were in the range of 96.7-103.6% for chicken sausage and 95.8-104.6% for pork sausage The established method for the determination of biogenic amines except for PUT from real samples was satisfactory.; A pre-column derivatization method for the simple, sensitive determination of biogenic amines using 10-ethyl-acridine-3-sulfonyl chloride (EASC) as labeling reagent with fluorescence detection and mass spectrometry (MS) identification has been developed. After pre-column derivatization, the labeled biogenic amines were separated on a Hypersil BDS-C18 column by gradient elution. The derivatives showed an intense protonated molecular ion corresponding m/z [M + H](+) in positive-ion mode. The collision-induced dissociation of protonated molecular ion formed specific fragment ions at m/z 196.5, m/z 222.7, m/z 224.4 and m/z 272.5, m/z 286.2. Satisfactory linear responses were observed at the concentration range of 0.02-10 mu mol L-1 with coefficients of > 0.9993. Detection limits obtained by the analysis of a derivatized standard containing 0.2 pmol of each biogenic amine, were from 20.22 to 109.2 fmol (at a signal-to-noise ratio of 3). The relative standard deviations of retention times and peak areas for each biogenic amine were < 0.96 and 3.22%, respectively. Recoveries except for PUT were in the range of 96.7-103.6% for chicken sausage and 95.8-104.6% for pork sausage The established method for the determination of biogenic amines except for PUT from real samples was satisfactory. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/40862 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Kang, Li,You, Jinmao,Sun, Zhiwei,et al. LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification[J]. CHROMATOGRAPHIA, Kang, L; You, JM; Sun, ZW; Wang, CY; Ji, ZY; Gao, YP; Suo, YR; Li, YL.LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification,CHROMATOGRAPHIA,2011,73(1-2):43-50,2011. |
APA | Kang, Li.,You, Jinmao.,Sun, Zhiwei.,Wang, Chengyan.,Ji, Zhongyin.,...&Li, Yulin.(2011).LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification.CHROMATOGRAPHIA. |
MLA | Kang, Li,et al."LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification".CHROMATOGRAPHIA (2011). |
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