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基于Solexa高通量测序的唐古特红景天(Rhodiola algida)微卫星信息分析
雷淑芸; 高庆波; 付鹏程; 杨慧玲; 陈世龙; 张发起
2014
Source Publication植物研究
ISSN1673-5102
Issue6Pages:829-834
Abstract利用所获得的Solexa高通量唐古特红景天转录组拼接EST序列进行微卫星位点的挖掘分析,期望为红景天属SSR标记的开发提供生物信息学依据。在得到的6552条EST序列中,三碱基最多,占总EST序列的41.50%;单核苷酸和二核苷酸重复类型的SSR含量相似,分别为27.76%和24.76%;二至六碱基微卫星分布密度与其对应的SSR含量成正比。在单核苷酸重复类型中,T和A重复类型最多,分别为总SSR的14.91%、12.70%,而G和C重复类型则很少;在二核苷酸重复类型中,AG重复类型最多,占总SSR的5.60%,GA和TC重复类型次之,分别为4.75%、4.72%;在三核苷酸重复类型中,GAA重复类型最多,为总SSR的1.85%,GAT次之,为1.79%,TTC、TCT、TCA、GGA、GCT、GAG重复类型间的SSR数相差不大;四、五、六核苷酸重复类型则很少。除五、六核苷酸重复类型外,其长度变化与其对应的重复类型碱基长度成反比;同种重复类型中,微卫星的长度与其对应的SSR数成反比。
Keyword唐古特红景天 高通量测序 微卫星
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/4092
Collection中国科学院西北高原生物研究所
Recommended Citation
GB/T 7714
雷淑芸,高庆波,付鹏程,等. 基于Solexa高通量测序的唐古特红景天(Rhodiola algida)微卫星信息分析[J]. 植物研究,2014(6):829-834.
APA 雷淑芸,高庆波,付鹏程,杨慧玲,陈世龙,&张发起.(2014).基于Solexa高通量测序的唐古特红景天(Rhodiola algida)微卫星信息分析.植物研究(6),829-834.
MLA 雷淑芸,et al."基于Solexa高通量测序的唐古特红景天(Rhodiola algida)微卫星信息分析".植物研究 .6(2014):829-834.
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