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Effects of emodin on the gene expression profiling of human breast carcinoma cells | |
Huang, Zhiwei ; Chen, Guichen ; Shi, Ping | |
2009 | |
发表期刊 | CANCER DETECTION AND PREVENTION ; Huang, ZW; Chen, GC; Shi, P.Effects of emodin on the gene expression profiling of human breast carcinoma cells,CANCER DETECTION AND PREVENTION,2009,32(4):286-291 |
摘要 | Background: The mechanism of emodin-mediated cell apoptosis has been investigated extensively in many types of human cancer cells. Our previous study demonstrated that emodin induced apoptosis through the decrease of Bcl-2/Bax ratio and the increase of cytoplasm cytochrome c concentration in human breast cancer BCap-37 cells. However, emodin's reaction to breast cancer cells remains elusive. Materials and methods: An apoptosis-associated cDNA microarray comprised of 458 known genes, namely, death receptors, calpains, death kinases, granzymes, DNA fragmentation proteins, caspases and Bcl-2 family, was used to determine the impact of emodin in breast cancer BCap-37 cells. Furthermore, the candidate emodin target genes were further evaluated via real-time quantitative PCR and Western blot analysis. Results: We found that gene expression profiling in human breast cancer BCap-37 cells was altered when exposed to emodin. Thirty of the unique genes that were either induced or repressed in response to emodin-induced apoptosis were also identified. A follow-up study characterized p53, emodin-induced gene, IGF-2, and emodin-repressed gene, and the downstream proteins were also seen as possible molecular targets of emodin. Conclusion: Data from this study provide novel evidence that emodin induces gene expression profiling changes, but has no effects on caspases. In addition, the p53 pathway may cooperate with the IGF-2 pathway, resulting in an emodin-induced apoptosis through disruption of the mitochondrial signaling pathway in BCap-37 cells. Published by Elsevier Ltd.; Background: The mechanism of emodin-mediated cell apoptosis has been investigated extensively in many types of human cancer cells. Our previous study demonstrated that emodin induced apoptosis through the decrease of Bcl-2/Bax ratio and the increase of cytoplasm cytochrome c concentration in human breast cancer BCap-37 cells. However, emodin's reaction to breast cancer cells remains elusive. Materials and methods: An apoptosis-associated cDNA microarray comprised of 458 known genes, namely, death receptors, calpains, death kinases, granzymes, DNA fragmentation proteins, caspases and Bcl-2 family, was used to determine the impact of emodin in breast cancer BCap-37 cells. Furthermore, the candidate emodin target genes were further evaluated via real-time quantitative PCR and Western blot analysis. Results: We found that gene expression profiling in human breast cancer BCap-37 cells was altered when exposed to emodin. Thirty of the unique genes that were either induced or repressed in response to emodin-induced apoptosis were also identified. A follow-up study characterized p53, emodin-induced gene, IGF-2, and emodin-repressed gene, and the downstream proteins were also seen as possible molecular targets of emodin. Conclusion: Data from this study provide novel evidence that emodin induces gene expression profiling changes, but has no effects on caspases. In addition, the p53 pathway may cooperate with the IGF-2 pathway, resulting in an emodin-induced apoptosis through disruption of the mitochondrial signaling pathway in BCap-37 cells. Published by Elsevier Ltd. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/41019 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Huang, Zhiwei,Chen, Guichen,Shi, Ping. Effects of emodin on the gene expression profiling of human breast carcinoma cells[J]. CANCER DETECTION AND PREVENTION, Huang, ZW; Chen, GC; Shi, P.Effects of emodin on the gene expression profiling of human breast carcinoma cells,CANCER DETECTION AND PREVENTION,2009,32(4):286-291,2009. |
APA | Huang, Zhiwei,Chen, Guichen,&Shi, Ping.(2009).Effects of emodin on the gene expression profiling of human breast carcinoma cells.CANCER DETECTION AND PREVENTION. |
MLA | Huang, Zhiwei,et al."Effects of emodin on the gene expression profiling of human breast carcinoma cells".CANCER DETECTION AND PREVENTION (2009). |
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