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Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.)
Lei, Yunting1; Zhao, Yanyan1,2; Yu, Feng1,2; Li, Yuan1,2; Dou, Quanwen1
2014-12-01
Source PublicationCONSERVATION GENETICS RESOURCES
ISSN1877-7252
Volume6Issue:4Pages:861-864
SubtypeArticle
AbstractSiberian wildrye, Elymus sibiricus L., is an important perennial forage crop in temperate regions of the world, especially in the Qinghai-Tibet plateau. However, it is now threatened by climate warming, excessive grazing, and damage to its natural habitat. A total of 53 polymorphic genomic-SSR markers were isolated and characterized in E. sibiricus. The number of alleles for these markers in 11 individuals varied from 2 to 5, with an average of 3.09 alleles per locus. Segregation analysis in an F-2 population revealed that the tested genomic-SSR markers had a co-dominant nature and Mendelian inheritance. Cross-species amplification tests showed that about half of the total E. sibiricus genomic-SSR markers could be effectively amplified in E. nutans. These SSR markers are the first characterized in E. sibiricus and will be useful for investigating genetic diversity and molecular-assisted breeding.; Siberian wildrye, Elymus sibiricus L., is an important perennial forage crop in temperate regions of the world, especially in the Qinghai-Tibet plateau. However, it is now threatened by climate warming, excessive grazing, and damage to its natural habitat. A total of 53 polymorphic genomic-SSR markers were isolated and characterized in E. sibiricus. The number of alleles for these markers in 11 individuals varied from 2 to 5, with an average of 3.09 alleles per locus. Segregation analysis in an F-2 population revealed that the tested genomic-SSR markers had a co-dominant nature and Mendelian inheritance. Cross-species amplification tests showed that about half of the total E. sibiricus genomic-SSR markers could be effectively amplified in E. nutans. These SSR markers are the first characterized in E. sibiricus and will be useful for investigating genetic diversity and molecular-assisted breeding.
KeywordSiberian Wildrye Elymus Sibiricus Genomic-ssr Polymorphism
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiodiversity & Conservation ; Genetics & Heredity
WOS SubjectBiodiversity Conservation ; Genetics & Heredity
WOS IDWOS:000344481900014
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/4172
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Lei, Yunting,Zhao, Yanyan,Yu, Feng,et al. Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.)[J]. CONSERVATION GENETICS RESOURCES,2014,6(4):861-864.
APA Lei, Yunting,Zhao, Yanyan,Yu, Feng,Li, Yuan,&Dou, Quanwen.(2014).Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.).CONSERVATION GENETICS RESOURCES,6(4),861-864.
MLA Lei, Yunting,et al."Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.)".CONSERVATION GENETICS RESOURCES 6.4(2014):861-864.
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