Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.) | |
| Lei, Yunting1; Zhao, Yanyan1,2; Yu, Feng1,2; Li, Yuan1,2; Dou, Quanwen1 | |
| 2014-12-01 | |
| 发表期刊 | CONSERVATION GENETICS RESOURCES
 |
| ISSN | 1877-7252 |
| 卷号 | 6期号:4页码:861-864 |
| 文章类型 | Article |
| 摘要 | Siberian wildrye, Elymus sibiricus L., is an important perennial forage crop in temperate regions of the world, especially in the Qinghai-Tibet plateau. However, it is now threatened by climate warming, excessive grazing, and damage to its natural habitat. A total of 53 polymorphic genomic-SSR markers were isolated and characterized in E. sibiricus. The number of alleles for these markers in 11 individuals varied from 2 to 5, with an average of 3.09 alleles per locus. Segregation analysis in an F-2 population revealed that the tested genomic-SSR markers had a co-dominant nature and Mendelian inheritance. Cross-species amplification tests showed that about half of the total E. sibiricus genomic-SSR markers could be effectively amplified in E. nutans. These SSR markers are the first characterized in E. sibiricus and will be useful for investigating genetic diversity and molecular-assisted breeding.; Siberian wildrye, Elymus sibiricus L., is an important perennial forage crop in temperate regions of the world, especially in the Qinghai-Tibet plateau. However, it is now threatened by climate warming, excessive grazing, and damage to its natural habitat. A total of 53 polymorphic genomic-SSR markers were isolated and characterized in E. sibiricus. The number of alleles for these markers in 11 individuals varied from 2 to 5, with an average of 3.09 alleles per locus. Segregation analysis in an F-2 population revealed that the tested genomic-SSR markers had a co-dominant nature and Mendelian inheritance. Cross-species amplification tests showed that about half of the total E. sibiricus genomic-SSR markers could be effectively amplified in E. nutans. These SSR markers are the first characterized in E. sibiricus and will be useful for investigating genetic diversity and molecular-assisted breeding. |
| 关键词 | Siberian Wildrye Elymus Sibiricus Genomic-ssr Polymorphism |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biodiversity & Conservation ; Genetics & Heredity |
| WOS类目 | Biodiversity Conservation ; Genetics & Heredity |
| WOS记录号 | WOS:000344481900014 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/4172 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lei, Yunting,Zhao, Yanyan,Yu, Feng,et al. Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.)[J]. CONSERVATION GENETICS RESOURCES,2014,6(4):861-864. |
| APA | Lei, Yunting,Zhao, Yanyan,Yu, Feng,Li, Yuan,&Dou, Quanwen.(2014).Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.).CONSERVATION GENETICS RESOURCES,6(4),861-864. |
| MLA | Lei, Yunting,et al."Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.)".CONSERVATION GENETICS RESOURCES 6.4(2014):861-864. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Development and char（164KB） | 开放获取 | CC BY-NC-SA | 浏览 下载 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论