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题名: Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.)
作者: Lei, Yunting1; Zhao, Yanyan1, 2; Yu, Feng1, 2; Li, Yuan1, 2; Dou, Quanwen1
刊名: CONSERVATION GENETICS RESOURCES
出版日期: 2014-12-01
卷号: 6, 期号:4, 页码:861-864
关键词: Siberian wildrye ; Elymus sibiricus ; Genomic-SSR ; Polymorphism
文章类型: Article
中文摘要: Siberian wildrye, Elymus sibiricus L., is an important perennial forage crop in temperate regions of the world, especially in the Qinghai-Tibet plateau. However, it is now threatened by climate warming, excessive grazing, and damage to its natural habitat. A total of 53 polymorphic genomic-SSR markers were isolated and characterized in E. sibiricus. The number of alleles for these markers in 11 individuals varied from 2 to 5, with an average of 3.09 alleles per locus. Segregation analysis in an F-2 population revealed that the tested genomic-SSR markers had a co-dominant nature and Mendelian inheritance. Cross-species amplification tests showed that about half of the total E. sibiricus genomic-SSR markers could be effectively amplified in E. nutans. These SSR markers are the first characterized in E. sibiricus and will be useful for investigating genetic diversity and molecular-assisted breeding.
英文摘要: Siberian wildrye, Elymus sibiricus L., is an important perennial forage crop in temperate regions of the world, especially in the Qinghai-Tibet plateau. However, it is now threatened by climate warming, excessive grazing, and damage to its natural habitat. A total of 53 polymorphic genomic-SSR markers were isolated and characterized in E. sibiricus. The number of alleles for these markers in 11 individuals varied from 2 to 5, with an average of 3.09 alleles per locus. Segregation analysis in an F-2 population revealed that the tested genomic-SSR markers had a co-dominant nature and Mendelian inheritance. Cross-species amplification tests showed that about half of the total E. sibiricus genomic-SSR markers could be effectively amplified in E. nutans. These SSR markers are the first characterized in E. sibiricus and will be useful for investigating genetic diversity and molecular-assisted breeding.
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biodiversity Conservation ; Genetics & Heredity
研究领域[WOS]: Biodiversity & Conservation ; Genetics & Heredity
收录类别: SCI
语种: 英语
WOS记录号: WOS:000344481900014
Citation statistics:
内容类型: 期刊论文
URI标识: http://210.75.249.4/handle/363003/4172
Appears in Collections:中国科学院西北高原生物研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Lei, YT; Zhao, YY; Yu, F; Li, Y; Dou, QW.Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.),CONSERVATION GENETICS RESOURCES,2014,6(4):861
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