Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry | |
| Zhao Xian-En1; Lu Tao1; Wei Na1; Zhu Shu-Yun1; You Jin-Mao1,2 | |
| 2014-11-01 | |
| 发表期刊 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| ISSN | 0253-3820 |
| 卷号 | 42期号:11页码:1629-1633 |
| 文章类型 | Article |
| 摘要 | Commercial Rhodamine B was first reported as a derivatization reagent labeling hydroxyl compound for mass spectrometry sensitization. In virtue of the strategy of ultrasonic-assisted dispersive liquid-liquid microextraction (UA-DLLME) combined with Rhodamine B derivatization, a new method of ultrahigh performance liquid chromatography triple quadrupole mass spectrometry (UHPLC-MS/MS) was developed for the sensitive determination of Panaxadiol (PD). UA-DLLME and derivatization conditions were investigated. PD in cosmetics and ginseng was extracted by UA-DLLME (150 mu L chloroform as extraction solvent, 500 mu L ethanol as disperser solvent, 3 min). Using Rhodamine B as precolumn derivatization reagent, the stable derivative of PD was obtained in acetonitrile at 70 degrees C for 30 min under microwave radiation (800 W) with the catalyst of N, N'-carbonyldiimidazole (CDI) and 4-dimethylaminopyridine (DMAP). And then the PD derivative was separated and detected within 3 min by multiple reaction monitoring (MRM) mode of UHPLC-MS/MS. The LOD was 4.0 ng L-1, and the LOQ was 15.0 ng L-1. This method had perfect linearity, precision and recovery results, and showed obvious advantages such as sensitivity and low matrix effect in comparison with the previously reported methods.; Commercial Rhodamine B was first reported as a derivatization reagent labeling hydroxyl compound for mass spectrometry sensitization. In virtue of the strategy of ultrasonic-assisted dispersive liquid-liquid microextraction (UA-DLLME) combined with Rhodamine B derivatization, a new method of ultrahigh performance liquid chromatography triple quadrupole mass spectrometry (UHPLC-MS/MS) was developed for the sensitive determination of Panaxadiol (PD). UA-DLLME and derivatization conditions were investigated. PD in cosmetics and ginseng was extracted by UA-DLLME (150 mu L chloroform as extraction solvent, 500 mu L ethanol as disperser solvent, 3 min). Using Rhodamine B as precolumn derivatization reagent, the stable derivative of PD was obtained in acetonitrile at 70 degrees C for 30 min under microwave radiation (800 W) with the catalyst of N, N'-carbonyldiimidazole (CDI) and 4-dimethylaminopyridine (DMAP). And then the PD derivative was separated and detected within 3 min by multiple reaction monitoring (MRM) mode of UHPLC-MS/MS. The LOD was 4.0 ng L-1, and the LOQ was 15.0 ng L-1. This method had perfect linearity, precision and recovery results, and showed obvious advantages such as sensitivity and low matrix effect in comparison with the previously reported methods. |
| 关键词 | Ultrasonic-assisted Dispersive Liquid-liquid Microextraction Rhodamine b Derivatization Mass Spectrometry Sensitization Panaxadiol |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 关键词[WOS] | FLUORESCENCE ; GINSENOSIDES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| WOS记录号 | WOS:000345116900006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/4175 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao Xian-En,Lu Tao,Wei Na,et al. Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2014,42(11):1629-1633. |
| APA | Zhao Xian-En,Lu Tao,Wei Na,Zhu Shu-Yun,&You Jin-Mao.(2014).Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,42(11),1629-1633. |
| MLA | Zhao Xian-En,et al."Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 42.11(2014):1629-1633. |
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