Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells | |
| Wang, Yi1; Nie, Fangyuan1; Ouyang, Jian2; Wang, Xiaoyan2; Ma, Xiaofeng1 | |
| 2014-10-01 | |
| 发表期刊 | TUMOR BIOLOGY
 |
| ISSN | 1010-4283 |
| 卷号 | 35期号:10页码:9563-9569 |
| 文章类型 | Article |
| 摘要 | Fatty acid synthase (FAS) is overexpressed in many human cancers including breast cancer and is considered to be a promising target for therapy. Sea buckthorn has long been used to treat a variety of maladies. Here, we investigated the inhibitory effect of sea buckthorn procyanidins (SBPs) isolated from the seeds of sea buckthorn on FAS and FAS overexpressed human breast cancer MDA-MB-231 cells. The FAS activity and FAS inhibition were measured by a spectrophotometer at 340 nm of nicotinamide adenine dinucleotide phosphate (NADPH) absorption. We found that SBP potently inhibited the activity of FAS with a half-inhibitory concentration (IC50) value of 0.087 mu g/ml. 3-4,5-Dimethylthiazol- 2-yl-2,3-diphenyl tetrazolium bromide (MTT) assay was used to test the cell viability. SBP reduced MDA-MB-231 cell viability with an IC50 value of 37.5 mu g/ml. Hoechst 33258/propidium iodide dual staining and flow cytometric analysis showed that SBP induced MDA-MB-231 cell apoptosis. SBP inhibited intracellular FAS activity with a dose-dependent manner. In addition, sodium palmitate could rescue the cell apoptosis induced by SBP. These results showed that SBP was a promising FAS inhibitor which could induce the apoptosis of MDA-MB-231 cells via inhibiting FAS. These findings suggested that SBP might be useful for preventing or treating breast cancer.; Fatty acid synthase (FAS) is overexpressed in many human cancers including breast cancer and is considered to be a promising target for therapy. Sea buckthorn has long been used to treat a variety of maladies. Here, we investigated the inhibitory effect of sea buckthorn procyanidins (SBPs) isolated from the seeds of sea buckthorn on FAS and FAS overexpressed human breast cancer MDA-MB-231 cells. The FAS activity and FAS inhibition were measured by a spectrophotometer at 340 nm of nicotinamide adenine dinucleotide phosphate (NADPH) absorption. We found that SBP potently inhibited the activity of FAS with a half-inhibitory concentration (IC50) value of 0.087 mu g/ml. 3-4,5-Dimethylthiazol- 2-yl-2,3-diphenyl tetrazolium bromide (MTT) assay was used to test the cell viability. SBP reduced MDA-MB-231 cell viability with an IC50 value of 37.5 mu g/ml. Hoechst 33258/propidium iodide dual staining and flow cytometric analysis showed that SBP induced MDA-MB-231 cell apoptosis. SBP inhibited intracellular FAS activity with a dose-dependent manner. In addition, sodium palmitate could rescue the cell apoptosis induced by SBP. These results showed that SBP was a promising FAS inhibitor which could induce the apoptosis of MDA-MB-231 cells via inhibiting FAS. These findings suggested that SBP might be useful for preventing or treating breast cancer. |
| 关键词 | Fatty Acid Synthase Inhibitor Sea Buckthorn Procyanidins Mda-mb-231 Cells Cell Apoptosis |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 关键词[WOS] | SEABUCKTHORN HIPPOPHAE-RHAMNOIDES ; INDUCED OXIDATIVE STRESS ; NF-KAPPA-B ; CANCER-CELLS ; EPIGALLOCATECHIN GALLATE ; ANTITUMOR-ACTIVITY ; MOLECULAR TARGETS ; NATURAL INHIBITOR ; PROSTATE-CANCER ; PALMITIC ACID |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Oncology |
| WOS类目 | Oncology |
| WOS记录号 | WOS:000344532700014 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/4181 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Qinghai Provinc, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yi,Nie, Fangyuan,Ouyang, Jian,et al. Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells[J]. TUMOR BIOLOGY,2014,35(10):9563-9569. |
| APA | Wang, Yi,Nie, Fangyuan,Ouyang, Jian,Wang, Xiaoyan,&Ma, Xiaofeng.(2014).Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells.TUMOR BIOLOGY,35(10),9563-9569. |
| MLA | Wang, Yi,et al."Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells".TUMOR BIOLOGY 35.10(2014):9563-9569. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Inhibitory effects o（780KB） | 开放获取 | CC BY-NC-SA | 浏览 下载 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论