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Development and characterization of eleven microsatellite markers for an endangered cavefish (Triplophysa rosa Chen and Yang, 2005) using 454 sequencing
Zhao, J.1; Zhao, K.2; Peng, Z.1
2014-10-01
发表期刊JOURNAL OF APPLIED ICHTHYOLOGY
ISSN0175-8659
卷号30期号:5页码:1056-1058
文章类型Article
摘要Triplophysa rosa is an endangered cave-dwelling and endemic fish species found only in Chongqing, southwestern China. The genetic data available for this species is very limited. Polymorphic microsatellites were identified in the genome of T. rosa using 454 sequencing. Of the 145 loci screened, 106 were amplified successfully and 11 showed polymorphic patterns. The number of alleles ranged from 2 to 6, and the observed and expected heterozygosity varied from 0.061 to 0.543 (mean = 0.349) and from 0.248 to 0.789 (mean = 0.551), respectively. The polymorphic information content (PIC) ranged from 0.215 to 0.744 (mean = 0.486), indicating moderate levels of polymorphism. In addition, cross-species amplification was tested for the 106 loci in Triplophysa moquensis, which showed a high level of transferability (76.4%), implying that the microsatellite markers developed here could be used effectively for other closely related species.; Triplophysa rosa is an endangered cave-dwelling and endemic fish species found only in Chongqing, southwestern China. The genetic data available for this species is very limited. Polymorphic microsatellites were identified in the genome of T. rosa using 454 sequencing. Of the 145 loci screened, 106 were amplified successfully and 11 showed polymorphic patterns. The number of alleles ranged from 2 to 6, and the observed and expected heterozygosity varied from 0.061 to 0.543 (mean = 0.349) and from 0.248 to 0.789 (mean = 0.551), respectively. The polymorphic information content (PIC) ranged from 0.215 to 0.744 (mean = 0.486), indicating moderate levels of polymorphism. In addition, cross-species amplification was tested for the 106 loci in Triplophysa moquensis, which showed a high level of transferability (76.4%), implying that the microsatellite markers developed here could be used effectively for other closely related species.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]LOACH ; CHINA
收录类别SCI
语种英语
WOS研究方向Fisheries ; Marine & Freshwater Biology
WOS类目Fisheries ; Marine & Freshwater Biology
WOS记录号WOS:000342991000035
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/4185
专题中国科学院西北高原生物研究所
作者单位1.Southwest Univ, Sch Life Sci, Minist Educ, Key Lab Freshwater Fish Reprod & Dev, Chongqing 400715, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Peoples R China
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Zhao, J.,Zhao, K.,Peng, Z.. Development and characterization of eleven microsatellite markers for an endangered cavefish (Triplophysa rosa Chen and Yang, 2005) using 454 sequencing[J]. JOURNAL OF APPLIED ICHTHYOLOGY,2014,30(5):1056-1058.
APA Zhao, J.,Zhao, K.,&Peng, Z..(2014).Development and characterization of eleven microsatellite markers for an endangered cavefish (Triplophysa rosa Chen and Yang, 2005) using 454 sequencing.JOURNAL OF APPLIED ICHTHYOLOGY,30(5),1056-1058.
MLA Zhao, J.,et al."Development and characterization of eleven microsatellite markers for an endangered cavefish (Triplophysa rosa Chen and Yang, 2005) using 454 sequencing".JOURNAL OF APPLIED ICHTHYOLOGY 30.5(2014):1056-1058.
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