Knowledge Management System of Northwest Institute of Plateau Biology, CAS
The detection of a de novo allele of the Glu-1Dx gene in wheat-rye hybrid offspring | |
Yuan, Zhongwei1; Liu, Miao1; Ouyang, Yuyuan1; Zeng, Xiaoxue1; Hao, Ming1; Zhang, Lianquan1; Ning, Shunzong1; Yan, Zehong1; Liu, Dengcai1,2 | |
2014-10-01 | |
发表期刊 | THEORETICAL AND APPLIED GENETICS |
ISSN | 0040-5752 |
卷号 | 127期号:10页码:2173-2182 |
文章类型 | Article |
摘要 | This study provides a link between a de novo gene and novel phenotype in wheat-rye hybrids that can be used as a model for induced de novo genetic variation. Wide hybridization can produce de novo DNA variation that may cause novel phenotypes. However, there is still a lack of specific links between changed genes and novel phenotypes in wide hybrids. The well-studied high-molecular-weight glutenin subunit (HMW-GS) genes in tribe Triticeae provide a useful model for addressing this issue. In this study, we investigated the feasibility of a wheat-rye hybridization method for inducing de novo phenotypes using the Glu-1Dx2.2 subunit as an example. We developed three hexaploid wheat lines with normal fertility and a Glu-1Dx2.2 variant, named Glu-1Dx2.2 (v) , derived from three F-1 hybrids. The wild-type Glu-1Dx2.2 has two direct repeats of 295 bp length separated by an intervening 101 bp in its central repetitive region. In the mutant Glu-1Dx2.2 (v) , one copy of the repeats and the intervening sequence were deleted, probably through homology-dependent illegitimate recombination (IR). This study provides a direct link between a de novo allele and novel phenotype. Our results indicate that the wheat-rye method may be a useful tool to induce de novo genetic variations that broaden the genetic diversity for wheat improvement.; This study provides a link between a de novo gene and novel phenotype in wheat-rye hybrids that can be used as a model for induced de novo genetic variation. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
关键词[WOS] | HIGH-MOLECULAR-WEIGHT ; SYNTHETIC HEXAPLOID WHEAT ; HMW GLUTENIN SUBUNITS ; GENOME EVOLUTION ; ILLEGITIMATE RECOMBINATION ; ALLOPOLYPLOID WHEAT ; WIDE HYBRIDIZATION ; TETRAPLOID WHEAT ; CROP IMPROVEMENT ; COMMON WHEAT |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Agriculture ; Plant Sciences ; Genetics & Heredity |
WOS类目 | Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture |
WOS记录号 | WOS:000342494500010 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/4189 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Sichuan Agr Univ, Triticeae Res Inst, Wenjiang 611130, Sichuan, Peoples R China 2.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Inst Plateau Biol, Xining 810001, Peoples R China |
推荐引用方式 GB/T 7714 | Yuan, Zhongwei,Liu, Miao,Ouyang, Yuyuan,et al. The detection of a de novo allele of the Glu-1Dx gene in wheat-rye hybrid offspring[J]. THEORETICAL AND APPLIED GENETICS,2014,127(10):2173-2182. |
APA | Yuan, Zhongwei.,Liu, Miao.,Ouyang, Yuyuan.,Zeng, Xiaoxue.,Hao, Ming.,...&Liu, Dengcai.(2014).The detection of a de novo allele of the Glu-1Dx gene in wheat-rye hybrid offspring.THEORETICAL AND APPLIED GENETICS,127(10),2173-2182. |
MLA | Yuan, Zhongwei,et al."The detection of a de novo allele of the Glu-1Dx gene in wheat-rye hybrid offspring".THEORETICAL AND APPLIED GENETICS 127.10(2014):2173-2182. |
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