Preparation of a Novel Glucuronomannan from Auricularia auricala and its Immunological Activity

NWIPB OpenIR

	Preparation of a Novel Glucuronomannan from Auricularia auricala and its Immunological Activity
	Li, Zonghong ; Jiang, Dan ; Bi, Hongtao ; Liu, Dazheng ; Jang, Sungju ; Zhou, Yifa
	2012-11-01
发表期刊	NATURAL PRODUCT COMMUNICATIONS ; Li, ZH; Jiang, D; Bi, HT; Liu, DZ; Jang, SJ; Zhou, YF.Preparation of a Novel Glucuronomannan from Auricularia auricala and its Immunological Activity,NATURAL PRODUCT COMMUNICATIONS,2012,7(11):1507-1510
摘要	A glucuronomannan (AA-4-H, Mw around 4 KDa) was prepared from the fruit bodies of Auricularia auricala by extraction with hot water, deproteination by Sevag reagent, stepwise precipitation with ethanol and partial acid hydrolysis. Monosaccharides analysis revealed that AA-4-H consisted of 91% mannose (Man) and 9% glucuronic acid (GlcA). FT-IR, NMR and methylation analyses indicated that AA-4-H is a branched glucuronomannan. Its main chains are composed of 1, 3-linked alpha-Manp, side chains are single alpha-Manp or alpha-GlcA residues attached to the O-2 and O-6 of Man residues of the main chains. Bioassay indicated that AA-4-H remarkably enhanced B lymphocyte proliferation and increased the production of nitric oxide of macrophages in vitro. Thus, glucuronomannan AA-4-H could be explored as a potential immunostimulation agent.; A glucuronomannan (AA-4-H, Mw around 4 KDa) was prepared from the fruit bodies of Auricularia auricala by extraction with hot water, deproteination by Sevag reagent, stepwise precipitation with ethanol and partial acid hydrolysis. Monosaccharides analysis revealed that AA-4-H consisted of 91% mannose (Man) and 9% glucuronic acid (GlcA). FT-IR, NMR and methylation analyses indicated that AA-4-H is a branched glucuronomannan. Its main chains are composed of 1, 3-linked alpha-Manp, side chains are single alpha-Manp or alpha-GlcA residues attached to the O-2 and O-6 of Man residues of the main chains. Bioassay indicated that AA-4-H remarkably enhanced B lymphocyte proliferation and increased the production of nitric oxide of macrophages in vitro. Thus, glucuronomannan AA-4-H could be explored as a potential immunostimulation agent.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/41938
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, Zonghong,Jiang, Dan,Bi, Hongtao,et al. Preparation of a Novel Glucuronomannan from Auricularia auricala and its Immunological Activity[J]. NATURAL PRODUCT COMMUNICATIONS, Li, ZH; Jiang, D; Bi, HT; Liu, DZ; Jang, SJ; Zhou, YF.Preparation of a Novel Glucuronomannan from Auricularia auricala and its Immunological Activity,NATURAL PRODUCT COMMUNICATIONS,2012,7(11):1507-1510,2012.
APA	Li, Zonghong,Jiang, Dan,Bi, Hongtao,Liu, Dazheng,Jang, Sungju,&Zhou, Yifa.(2012).Preparation of a Novel Glucuronomannan from Auricularia auricala and its Immunological Activity.NATURAL PRODUCT COMMUNICATIONS.
MLA	Li, Zonghong,et al."Preparation of a Novel Glucuronomannan from Auricularia auricala and its Immunological Activity".NATURAL PRODUCT COMMUNICATIONS (2012).