OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC

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	OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC
	Dang, Jun 1,2; Chen, Chen 2; Shao, Yun 2; Mei, Lijuan 2; Zhang, Huaigang 2; Zhong, Zhengsheng 3; Wang, Qilan 2; Tao, Yanduo 2
	2014-08-27
发表期刊	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN	1082-6076
卷号	37 期号:14 页码:1940-1952
文章类型	Article
摘要	Extraction time, ratio of methanol aqueous solution to material weight, and methanol concentration for three factor and three level response surface methodology (RSM) was used to study the optimum extraction technology of gentiopicroside from Gentiana Straminea Maxim. The analysis was performed on a high performance liquid chromatography (HPLC) system with gentiopicroside peak areas as response values. A ternary quadratic polynomial model with good correlation (R-2=0.9786) was constructed and employed to guide the gentiopicroside extraction process. The optimum extraction conditions were an optimized extract time of 79.4min, a ratio of methanol aqueous solution to material weight of 23.7mL/g, methanol concentration of 92.6%, and three times extraction. Under the optimization extraction technology conditions, the predictive value of gentiopicroside peak area of the sample solution was 2120.6, and only 2.03% differ from the mean measured value 2164.6, and the optimized mean extraction yield of gentiopicroside was 78.3mg center dot g(-1). Through solvent distribution method crystallization of the extractive from response surface experiments, the white crystalline compound gentiopicroside was prepared and its purity was greater than 95%.; Extraction time, ratio of methanol aqueous solution to material weight, and methanol concentration for three factor and three level response surface methodology (RSM) was used to study the optimum extraction technology of gentiopicroside from Gentiana Straminea Maxim. The analysis was performed on a high performance liquid chromatography (HPLC) system with gentiopicroside peak areas as response values. A ternary quadratic polynomial model with good correlation (R-2=0.9786) was constructed and employed to guide the gentiopicroside extraction process. The optimum extraction conditions were an optimized extract time of 79.4min, a ratio of methanol aqueous solution to material weight of 23.7mL/g, methanol concentration of 92.6%, and three times extraction. Under the optimization extraction technology conditions, the predictive value of gentiopicroside peak area of the sample solution was 2120.6, and only 2.03% differ from the mean measured value 2164.6, and the optimized mean extraction yield of gentiopicroside was 78.3mg center dot g(-1). Through solvent distribution method crystallization of the extractive from response surface experiments, the white crystalline compound gentiopicroside was prepared and its purity was greater than 95%.
关键词	Peak Area Extraction Process Gentiana Straminea Maxim. Gentiopicroside Response Surface Methodology Hplc
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
关键词[WOS]	ASSISTED EXTRACTION ; SECOIRIDOIDS ; MACROPHYLLA
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000334067600003
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/4201
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810008, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Dang, Jun,Chen, Chen,Shao, Yun,et al. OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2014,37(14):1940-1952.
APA	Dang, Jun.,Chen, Chen.,Shao, Yun.,Mei, Lijuan.,Zhang, Huaigang.,...&Tao, Yanduo.(2014).OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,37(14),1940-1952.
MLA	Dang, Jun,et al."OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROM GENTIANA STRAMINEA MAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 37.14(2014):1940-1952.