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A novel method for trace aldehyde determination in foodstuffs based on fluorescence labeling by HPLC with fluorescence detection and mass spectrometric identification
Wang, Wenli1; Li, Guoliang1; Ji, Zhongyin1,2; Hu, Na2; You, Jinmao1,2
2014-08-01
Source PublicationFOOD ANALYTICAL METHODS
ISSN1936-9751
Volume7Issue:7Pages:1546-1556
SubtypeArticle
AbstractA stable, effective, sensitive and selective method for simultaneous determination of 11 aldehydes in foodstuffs using a novel fluorescence-labeling reagent 2-(12-benzo[b]acridin-5-(12H)-yl)-acetohydrazide (BAAH) has been developed by HPLC with fluorescence detection and mass spectrometric identification. Response surface methodology was employed to optimize the derivatization reaction between BAAH and aldehydes. The completed separation of the 11 aldehydes was achieved in as little as 18 min on a reversed-phase Hypersil BDS C-8 column with aqueous acetonitrile as mobile phase in conjunction with a binary gradient elution. Excellent linear coefficients were found to be of > 0.9994. This method also showed excellent reproducibility and offered the low detection limits of 0.21-0.58 nM (at a signal-to-noise ratio of 3). The developed method was successfully applied to analyze aldehydes in various foodstuffs and exhibited satisfactory applicability.; A stable, effective, sensitive and selective method for simultaneous determination of 11 aldehydes in foodstuffs using a novel fluorescence-labeling reagent 2-(12-benzo[b]acridin-5-(12H)-yl)-acetohydrazide (BAAH) has been developed by HPLC with fluorescence detection and mass spectrometric identification. Response surface methodology was employed to optimize the derivatization reaction between BAAH and aldehydes. The completed separation of the 11 aldehydes was achieved in as little as 18 min on a reversed-phase Hypersil BDS C-8 column with aqueous acetonitrile as mobile phase in conjunction with a binary gradient elution. Excellent linear coefficients were found to be of > 0.9994. This method also showed excellent reproducibility and offered the low detection limits of 0.21-0.58 nM (at a signal-to-noise ratio of 3). The developed method was successfully applied to analyze aldehydes in various foodstuffs and exhibited satisfactory applicability.
KeywordAldehydes Hplc-fld-ms/ms Pre-column Derivatization Response Surface Methodology Foodstuffs
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; SOLID-PHASE EXTRACTION ; POLYCYCLIC AROMATIC-HYDROCARBONS ; CARBONYL-COMPOUNDS ; ALIPHATIC-ALDEHYDES ; GAS-CHROMATOGRAPHY ; SENSORY ANALYSIS ; DERIVATIZATION ; WINE ; 2,4-DINITROPHENYLHYDRAZINE
Indexed BySCI
Language英语
WOS Research AreaFood Science & Technology
WOS SubjectFood Science & Technology
WOS IDWOS:000338447800024
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/4207
Collection中国科学院西北高原生物研究所
Affiliation1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China
Recommended Citation
GB/T 7714
Wang, Wenli,Li, Guoliang,Ji, Zhongyin,et al. A novel method for trace aldehyde determination in foodstuffs based on fluorescence labeling by HPLC with fluorescence detection and mass spectrometric identification[J]. FOOD ANALYTICAL METHODS,2014,7(7):1546-1556.
APA Wang, Wenli,Li, Guoliang,Ji, Zhongyin,Hu, Na,&You, Jinmao.(2014).A novel method for trace aldehyde determination in foodstuffs based on fluorescence labeling by HPLC with fluorescence detection and mass spectrometric identification.FOOD ANALYTICAL METHODS,7(7),1546-1556.
MLA Wang, Wenli,et al."A novel method for trace aldehyde determination in foodstuffs based on fluorescence labeling by HPLC with fluorescence detection and mass spectrometric identification".FOOD ANALYTICAL METHODS 7.7(2014):1546-1556.
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