DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION

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	DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION
	Chen, Xiangming 1,2,3; Dong, Xiuli 2; Suo, Yourui 1; You, Jinmao 1,4
	2014-07-03
发表期刊	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN	1082-6076
卷号	37 期号:11 页码:1548-1557
文章类型	Article
摘要	A highly sensitive method for the determination of free fatty acids (FFAs) using high-performance liquid chromatography (HPLC) with fluorescence detection has been developed. Twenty-six FFAs (C5-C30) were derivatized by a novel pre-column derivatization reagent 2-(11H-benzo[a]carbazol-11-yl)-ethyl-4-methyl- benzenesulphonate (BCETS). Derivatives were sufficiently stable to be efficiently analyzed by HPLC. All fatty acid derivatives were separated on a hypersil BDS-C8 column in conjunction with a gradient elution with a good baseline resolution. The identification was carried out by post-column online mass spectrometry with atmospheric pressure chemical ionization (APCI) in positive-ion detection mode. The established method exhibited good selectivity and excellent repeatability. The RSD (n=6) for each fatty acid derivative was <2.8%, and detection limit (at signal-to-noise of 3:1) was 15-64 fmol. The method was used to quantify FFAs in Cortex Lycii sample with satisfactory results.; A highly sensitive method for the determination of free fatty acids (FFAs) using high-performance liquid chromatography (HPLC) with fluorescence detection has been developed. Twenty-six FFAs (C5-C30) were derivatized by a novel pre-column derivatization reagent 2-(11H-benzo[a]carbazol-11-yl)-ethyl-4-methyl- benzenesulphonate (BCETS). Derivatives were sufficiently stable to be efficiently analyzed by HPLC. All fatty acid derivatives were separated on a hypersil BDS-C8 column in conjunction with a gradient elution with a good baseline resolution. The identification was carried out by post-column online mass spectrometry with atmospheric pressure chemical ionization (APCI) in positive-ion detection mode. The established method exhibited good selectivity and excellent repeatability. The RSD (n=6) for each fatty acid derivative was <2.8%, and detection limit (at signal-to-noise of 3:1) was 15-64 fmol. The method was used to quantify FFAs in Cortex Lycii sample with satisfactory results.
关键词	Cortex Lycii Pre-column Derivatization Fatty Acids Hplc/ms Fluorescence Detection
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
关键词[WOS]	CARBOXYLIC-ACIDS ; DERIVATIZATION REAGENT ; LABELING REAGENT ; FLUOROMETRIC DETECTION ; GAS-CHROMATOGRAPHY ; BIOLOGICAL SAMPLES ; HPLC ; 9-ANTHRYLDIAZOMETHANE ; MS ; METABOLITES
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000333991500005
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/4210
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining 810001, Qinghai, Peoples R China 2.Binzhou Med Univ, Coll Pharm, Yantai, Shandong, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xiangming,Dong, Xiuli,Suo, Yourui,et al. DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2014,37(11):1548-1557.
APA	Chen, Xiangming,Dong, Xiuli,Suo, Yourui,&You, Jinmao.(2014).DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,37(11),1548-1557.
MLA	Chen, Xiangming,et al."DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 37.11(2014):1548-1557.