Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk

NWIPB OpenIR

	Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk
	Hu, Xi-Gui ; Wu, Bi-Hua ; Liu, Deng-Cai ; Wei, Yu-Ming ; Gao, Shi-Bin ; Zhen, You-Liang ; Wu, BH (reprint author), Sichuan Agr Univ, Triticeae Res Inst, Wenjiang 611130, Peoples R China.
	2013-02-15
发表期刊	JOURNAL OF PLANT PHYSIOLOGY ; Hu, XG; Wu, BH; Liu, DC; Wei, YM; Gao, SB; Zhen, YL.Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk,JOURNAL OF PLANT PHYSIOLOGY,2013,170(3):330-337
摘要	NAM is an important domestication gene and valuable to enhance grain protein contents (GPCs) of modern wheat cultivars. In the present study, 12 NAM-G1 genes in Triticum timopheevii Zhuk. (AAGG, 2n = 4x = 28) were cloned. These genes had the same length of 1546 bp including two introns and three exons, and encoded a polypeptide of 407 amino acid residues which contained a N-terminal NAC domain with five sub-domains, and a C-terminal transcriptional activation region (TAR). They were highly similar to the previously published functional NAM-B1 gene DQ871219 from T. turgidum ssp. dicoccoides Korn. (AABB, 2n = 4x = 28) in both the nucleotide and protein sequence's, with a very high identity of 99.5%. The differences among the 12 NAM-G1 genes resulted from 17 SNPs including 14 transitions and 3 transversions. They had outstandingly different expression levels in qRT-PCR. And, their relative expression quantities were significantly positively correlated with GPC of the accessions. In addition, the difference in amino acid sequences of the NAM-G1 genes may also affect the GPC variation. Crown Copyright (C) 2012 Published by Elsevier GmbH. All rights reserved.; NAM is an important domestication gene and valuable to enhance grain protein contents (GPCs) of modern wheat cultivars. In the present study, 12 NAM-G1 genes in Triticum timopheevii Zhuk. (AAGG, 2n = 4x = 28) were cloned. These genes had the same length of 1546 bp including two introns and three exons, and encoded a polypeptide of 407 amino acid residues which contained a N-terminal NAC domain with five sub-domains, and a C-terminal transcriptional activation region (TAR). They were highly similar to the previously published functional NAM-B1 gene DQ871219 from T. turgidum ssp. dicoccoides Korn. (AABB, 2n = 4x = 28) in both the nucleotide and protein sequence's, with a very high identity of 99.5%. The differences among the 12 NAM-G1 genes resulted from 17 SNPs including 14 transitions and 3 transversions. They had outstandingly different expression levels in qRT-PCR. And, their relative expression quantities were significantly positively correlated with GPC of the accessions. In addition, the difference in amino acid sequences of the NAM-G1 genes may also affect the GPC variation. Crown Copyright (C) 2012 Published by Elsevier GmbH. All rights reserved.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/42213
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Hu, Xi-Gui,Wu, Bi-Hua,Liu, Deng-Cai,et al. Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk[J]. JOURNAL OF PLANT PHYSIOLOGY, Hu, XG; Wu, BH; Liu, DC; Wei, YM; Gao, SB; Zhen, YL.Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk,JOURNAL OF PLANT PHYSIOLOGY,2013,170(3):330-337,2013.
APA	Hu, Xi-Gui.,Wu, Bi-Hua.,Liu, Deng-Cai.,Wei, Yu-Ming.,Gao, Shi-Bin.,...&Wu, BH .(2013).Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk.JOURNAL OF PLANT PHYSIOLOGY.
MLA	Hu, Xi-Gui,et al."Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk".JOURNAL OF PLANT PHYSIOLOGY (2013).