One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography

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	One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography
	Liu, Yongling 1,2; Wang, Ping 1,2; Chen, Tao 1; Jia, Jing 1; Sun, Jing 1; You, Jinmao 1; Li, Yulin 1
	2014-05-03
发表期刊	SEPARATION SCIENCE AND TECHNOLOGY
ISSN	0149-6395
卷号	49 期号:7 页码:1119-1124
文章类型	Article
摘要	High-speed counter-current chromatography (HSCCC) was successfully applied to the isolation and purification of four xanthone glycosides from Halenia elliptica, a plant widely used in traditional Tibetan medicine. The introduction of HSCCC greatly improved the efficiency of compounds preparation from Halenia elliptica. The following were obtained from 100 mg of crude sample in one-step separation: 2.5 mg of 1-O-primeverosyl-2,3,4,5,7-pentamethoxyxanthone, 7.0 mg of 1-O-primeverosyl-2,3,4,7- tetramethoxyxanthone, 10.0 mg of 1-O-primeverosyl-2,3,5-trimethoxyxanthone (demethyoxyhaleniaside), and 8.5 mg of 1-O-primeverosyl-2,3,4,5-tetramethoxyxanthone. HPLC analysis showed that each target compound had a purity of over 98%, and UV, H-1 NMR, and C-13 NMR data confirmed the component chemical structures.; High-speed counter-current chromatography (HSCCC) was successfully applied to the isolation and purification of four xanthone glycosides from Halenia elliptica, a plant widely used in traditional Tibetan medicine. The introduction of HSCCC greatly improved the efficiency of compounds preparation from Halenia elliptica. The following were obtained from 100 mg of crude sample in one-step separation: 2.5 mg of 1-O-primeverosyl-2,3,4,5,7-pentamethoxyxanthone, 7.0 mg of 1-O-primeverosyl-2,3,4,7- tetramethoxyxanthone, 10.0 mg of 1-O-primeverosyl-2,3,5-trimethoxyxanthone (demethyoxyhaleniaside), and 8.5 mg of 1-O-primeverosyl-2,3,4,5-tetramethoxyxanthone. HPLC analysis showed that each target compound had a purity of over 98%, and UV, H-1 NMR, and C-13 NMR data confirmed the component chemical structures.
关键词	Halenia Elliptica High-speed Counter-current Chromatography Xanthone Glycosides
WOS标题词	Science & Technology ; Physical Sciences ; Technology
关键词[WOS]	SEPARATION ; GLUCOSIDES
收录类别	SCI
语种	英语
WOS研究方向	Chemistry ; Engineering
WOS类目	Chemistry, Multidisciplinary ; Engineering, Chemical
WOS记录号	WOS:000335214100018
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/4232
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yongling,Wang, Ping,Chen, Tao,et al. One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography[J]. SEPARATION SCIENCE AND TECHNOLOGY,2014,49(7):1119-1124.
APA	Liu, Yongling.,Wang, Ping.,Chen, Tao.,Jia, Jing.,Sun, Jing.,...&Li, Yulin.(2014).One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography.SEPARATION SCIENCE AND TECHNOLOGY,49(7),1119-1124.
MLA	Liu, Yongling,et al."One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography".SEPARATION SCIENCE AND TECHNOLOGY 49.7(2014):1119-1124.