Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC | |
| Chen, Chen1; Zhao, Xiao-Hui1,2,3; Yue, Hui-Lan1; Li, Yu-Lin1; Chen, Tao1 | |
| 2014-05-01 | |
| 发表期刊 | JOURNAL OF CHROMATOGRAPHIC SCIENCE
 |
| ISSN | 0021-9665 |
| 卷号 | 52期号:5页码:395-399 |
| 文章类型 | Article |
| 摘要 | An effective high-speed counter-current chromatography method was established for the preparative isolation and purification of two phenyl-propanoid glycosides from the Tibetan medicinal plant Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong. With a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:3:4:5, v/v), 40 mg of an extract of Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong was separated to yield 20 mg of verbascoside and 18 mg of isoacteoside, with purity values of 97 and 98%, respectively. The chemical structures of these two components were identified by proton and carbon nuclear magnetic resonance. In addition, the antioxidant activity of the two phenylpropanoid glycosides was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH)-high-performance liquid chromatography, and the results showed that the two components exhibited strong antioxidant DPPH radical scavenging activity, with IC50 values of 15.6 and 18.9 mg/mL, respectively.; An effective high-speed counter-current chromatography method was established for the preparative isolation and purification of two phenyl-propanoid glycosides from the Tibetan medicinal plant Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong. With a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:3:4:5, v/v), 40 mg of an extract of Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong was separated to yield 20 mg of verbascoside and 18 mg of isoacteoside, with purity values of 97 and 98%, respectively. The chemical structures of these two components were identified by proton and carbon nuclear magnetic resonance. In addition, the antioxidant activity of the two phenylpropanoid glycosides was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH)-high-performance liquid chromatography, and the results showed that the two components exhibited strong antioxidant DPPH radical scavenging activity, with IC50 values of 15.6 and 18.9 mg/mL, respectively. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 关键词[WOS] | COUNTER-CURRENT CHROMATOGRAPHY ; PHENYLETHANOID GLYCOSIDES ; CISTANCHE-DESERTICOLA ; PURIFICATION ; ACTEOSIDE ; ISOACTEOSIDE ; EXTRACTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000342734600004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/4233 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Key Lab Plateau Crop Germplasm Innovat & Utilizat, State Key Lab Breeding Base, Xining 810016, Peoples R China 3.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Chen,Zhao, Xiao-Hui,Yue, Hui-Lan,et al. Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2014,52(5):395-399. |
| APA | Chen, Chen,Zhao, Xiao-Hui,Yue, Hui-Lan,Li, Yu-Lin,&Chen, Tao.(2014).Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC.JOURNAL OF CHROMATOGRAPHIC SCIENCE,52(5),395-399. |
| MLA | Chen, Chen,et al."Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC".JOURNAL OF CHROMATOGRAPHIC SCIENCE 52.5(2014):395-399. |
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