Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Inhibition of inducible nitric oxide synthase expression and nitric oxide production in plateau pika (Ochotona curzoniae) at high altitude on Qinghai-Tibet Plateau | |
| Xie, Ling1,2,5; Zhang, Xuze1; Qi, Delin3; Guo, Xinyi1,5; Pang, Bo1,5; Du, Yurong4; Zou, Xiaoyan1; Guo, Songchang1,2; Zhao, Xinquan1 | |
| 2014-04-30 | |
| 发表期刊 | NITRIC OXIDE-BIOLOGY AND CHEMISTRY
 |
| ISSN | 1089-8603 |
| 卷号 | 38页码:38-44 |
| 文章类型 | Article |
| 摘要 | Nitric oxide (NO), a potent vasodilator, plays an important role in preventing hypoxia induced pulmonary hypertension. Endogenous NO is synthesized by nitric oxide synthases (NOSs) from L-arginine. In mammals, three different NOSs have been identified, including neuronal NOS (nNOS), endothelial NOS (eNOS) and inducible NOS (iNOS). Plateau pika (Ochotona curzoniae) is a typical hypoxia tolerant mammal that lives at 3000-5000 m above sea level on the Qinghai-Tibet Plateau. The aim of this study was to investigate whether NOS expression and NO production are regulated by chronic hypoxia in plateau pika. Quantitative real-time PCR and western blot analyses were conducted to quantify relative abundances of iNOS and eNOS transcripts and proteins in the lung tissues of plateau pikas at different altitudes (4550, 3950 and 3200 m). Plasma NO metabolites, nitrite/nitrate (NOx-) levels were also examined by Ion chromatography to determine the correlation between NO production and altitude level. The results revealed that iNOS transcript levels were significantly lower in animals at high altitudes (decreased by 53% and 57% at altitude of 3950 and 4550 m compared with that at 3200 m). Similar trends in iNOS protein abundances were observed (26% and 41% at 3950 and 4550 m comparing with at 3200 m). There were no significant differences in eNOS mRNA and protein levels in the pika lungs among different altitudes. The plasma NO levels of the plateau pikas at high altitudes significantly decreased (1.65 +/- 0.19 mu g/mL at 3200 m to 0.44 +/- 0.03 mu g/mL at 3950 m and 0.24 +/- 0.01 mu g/mL at 4550 m). This is the first evidence describing the effects of chronic hypoxia on NOS expression and NO levels. in the plateau pika in high altitude adaptation. We conclude that iNOS expression and NO production are suppressed at high altitudes, and the lower NO concentration at high altitudes may serve crucial roles for helping the plateau pika to survive at hypoxic environment. (c) 2014 Elsevier Inc. All rights reserved.; Nitric oxide (NO), a potent vasodilator, plays an important role in preventing hypoxia induced pulmonary hypertension. Endogenous NO is synthesized by nitric oxide synthases (NOSs) from L-arginine. In mammals, three different NOSs have been identified, including neuronal NOS (nNOS), endothelial NOS (eNOS) and inducible NOS (iNOS). Plateau pika (Ochotona curzoniae) is a typical hypoxia tolerant mammal that lives at 3000-5000 m above sea level on the Qinghai-Tibet Plateau. The aim of this study was to investigate whether NOS expression and NO production are regulated by chronic hypoxia in plateau pika. Quantitative real-time PCR and western blot analyses were conducted to quantify relative abundances of iNOS and eNOS transcripts and proteins in the lung tissues of plateau pikas at different altitudes (4550, 3950 and 3200 m). Plasma NO metabolites, nitrite/nitrate (NOx-) levels were also examined by Ion chromatography to determine the correlation between NO production and altitude level. The results revealed that iNOS transcript levels were significantly lower in animals at high altitudes (decreased by 53% and 57% at altitude of 3950 and 4550 m compared with that at 3200 m). Similar trends in iNOS protein abundances were observed (26% and 41% at 3950 and 4550 m comparing with at 3200 m). There were no significant differences in eNOS mRNA and protein levels in the pika lungs among different altitudes. The plasma NO levels of the plateau pikas at high altitudes significantly decreased (1.65 +/- 0.19 mu g/mL at 3200 m to 0.44 +/- 0.03 mu g/mL at 3950 m and 0.24 +/- 0.01 mu g/mL at 4550 m). This is the first evidence describing the effects of chronic hypoxia on NOS expression and NO levels. in the plateau pika in high altitude adaptation. We conclude that iNOS expression and NO production are suppressed at high altitudes, and the lower NO concentration at high altitudes may serve crucial roles for helping the plateau pika to survive at hypoxic environment. (c) 2014 Elsevier Inc. All rights reserved. |
| 关键词 | Inducible Nitric Oxide Synthase Endothelial Nitric Oxide Synthase Nitric Oxide Plateau Pika Qinghai-tibet Plateau |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 关键词[WOS] | HYPOXIC PULMONARY VASOCONSTRICTION ; ARTERY ENDOTHELIAL-CELLS ; GENE-EXPRESSION ; HYDROGEN-SULFIDE ; DIFFERENT MECHANISMS ; UNANESTHETIZED RATS ; RESPONSIVE ELEMENT ; RESPIRATORY SYSTEM ; RELAXING FACTOR ; OXYGEN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS记录号 | WOS:000335287800006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/4239 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China 2.Key Lab Crop Mol Breeding, Xining, Qinghai Provinc, Peoples R China 3.Qinghai Univ, Coll Agr & Anim Husb, Dept Anim Sci, Xining, Peoples R China 4.Qinghai Normal Univ, Sch Life & Geog Sci, Xining, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Ling,Zhang, Xuze,Qi, Delin,et al. Inhibition of inducible nitric oxide synthase expression and nitric oxide production in plateau pika (Ochotona curzoniae) at high altitude on Qinghai-Tibet Plateau[J]. NITRIC OXIDE-BIOLOGY AND CHEMISTRY,2014,38:38-44. |
| APA | Xie, Ling.,Zhang, Xuze.,Qi, Delin.,Guo, Xinyi.,Pang, Bo.,...&Zhao, Xinquan.(2014).Inhibition of inducible nitric oxide synthase expression and nitric oxide production in plateau pika (Ochotona curzoniae) at high altitude on Qinghai-Tibet Plateau.NITRIC OXIDE-BIOLOGY AND CHEMISTRY,38,38-44. |
| MLA | Xie, Ling,et al."Inhibition of inducible nitric oxide synthase expression and nitric oxide production in plateau pika (Ochotona curzoniae) at high altitude on Qinghai-Tibet Plateau".NITRIC OXIDE-BIOLOGY AND CHEMISTRY 38(2014):38-44. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Inhibition of induci（596KB） | 开放获取 | CC BY-NC-SA | 浏览 下载 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论