Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells

NWIPB OpenIR

	Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells
	Wang, Yi ; Nie, Fangyuan ; Ouyang, Jian ; Wang, Xiaoyan ; Ma, Xiaofeng
	2014-10-01
发表期刊	TUMOR BIOLOGY ; Wang, Y; Nie, FY; Ouyang, J; Wang, XY; Ma, XF.Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells,TUMOR BIOLOGY,2014,35(10):9563
摘要	Fatty acid synthase (FAS) is overexpressed in many human cancers including breast cancer and is considered to be a promising target for therapy. Sea buckthorn has long been used to treat a variety of maladies. Here, we investigated the inhibitory effect of sea buckthorn procyanidins (SBPs) isolated from the seeds of sea buckthorn on FAS and FAS overexpressed human breast cancer MDA-MB-231 cells. The FAS activity and FAS inhibition were measured by a spectrophotometer at 340 nm of nicotinamide adenine dinucleotide phosphate (NADPH) absorption. We found that SBP potently inhibited the activity of FAS with a half-inhibitory concentration (IC50) value of 0.087 mu g/ml. 3-4,5-Dimethylthiazol- 2-yl-2,3-diphenyl tetrazolium bromide (MTT) assay was used to test the cell viability. SBP reduced MDA-MB-231 cell viability with an IC50 value of 37.5 mu g/ml. Hoechst 33258/propidium iodide dual staining and flow cytometric analysis showed that SBP induced MDA-MB-231 cell apoptosis. SBP inhibited intracellular FAS activity with a dose-dependent manner. In addition, sodium palmitate could rescue the cell apoptosis induced by SBP. These results showed that SBP was a promising FAS inhibitor which could induce the apoptosis of MDA-MB-231 cells via inhibiting FAS. These findings suggested that SBP might be useful for preventing or treating breast cancer.; Fatty acid synthase (FAS) is overexpressed in many human cancers including breast cancer and is considered to be a promising target for therapy. Sea buckthorn has long been used to treat a variety of maladies. Here, we investigated the inhibitory effect of sea buckthorn procyanidins (SBPs) isolated from the seeds of sea buckthorn on FAS and FAS overexpressed human breast cancer MDA-MB-231 cells. The FAS activity and FAS inhibition were measured by a spectrophotometer at 340 nm of nicotinamide adenine dinucleotide phosphate (NADPH) absorption. We found that SBP potently inhibited the activity of FAS with a half-inhibitory concentration (IC50) value of 0.087 mu g/ml. 3-4,5-Dimethylthiazol- 2-yl-2,3-diphenyl tetrazolium bromide (MTT) assay was used to test the cell viability. SBP reduced MDA-MB-231 cell viability with an IC50 value of 37.5 mu g/ml. Hoechst 33258/propidium iodide dual staining and flow cytometric analysis showed that SBP induced MDA-MB-231 cell apoptosis. SBP inhibited intracellular FAS activity with a dose-dependent manner. In addition, sodium palmitate could rescue the cell apoptosis induced by SBP. These results showed that SBP was a promising FAS inhibitor which could induce the apoptosis of MDA-MB-231 cells via inhibiting FAS. These findings suggested that SBP might be useful for preventing or treating breast cancer.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/42429
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Yi,Nie, Fangyuan,Ouyang, Jian,et al. Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells[J]. TUMOR BIOLOGY, Wang, Y; Nie, FY; Ouyang, J; Wang, XY; Ma, XF.Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells,TUMOR BIOLOGY,2014,35(10):9563,2014.
APA	Wang, Yi,Nie, Fangyuan,Ouyang, Jian,Wang, Xiaoyan,&Ma, Xiaofeng.(2014).Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells.TUMOR BIOLOGY.
MLA	Wang, Yi,et al."Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells".TUMOR BIOLOGY (2014).