Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes | |
Ma, Chengjun1; Wang, Jinjun1; Chu, Hongmei2; Zhang, Xiaoxiao1; Wang, Zhenhua1; Wang, Honglun3; Li, Gang1,3 | |
2014-03-01 | |
发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
ISSN | 1422-0067 |
卷号 | 15期号:3页码:3481-3494 |
文章类型 | Article |
摘要 | Aporphine alkaloids from the leaves of Nelumbo nucifera Gaertn are substances of great interest because of their important pharmacological activities, particularly anti-diabetic, anti-obesity, anti-hyperlipidemic, anti-oxidant, and anti-HIV's activities. In order to produce large amounts of pure alkaloid for research purposes, a novel method using high-speed counter-current chromatography (HSCCC) was developed. Without any initial cleanup steps, four main aporphine alkaloids, including 2-hydroxy-1-methoxyaporphine, pronuciferine, nuciferine and roemerine were successfully purified from the crude extract by HSCCC in one step. The separation was performed with a simple two-phase solvent system composed of n-hexane-ethyl acetate-methanol-acetonitrile-water (5:3:3:2.5:5, v/v/v/v/v). In each operation, 100 mg crude extracts was separated and yielded 6.3 mg of 2-hydroxy-1-methoxyaporphine (95.1% purity), 1.1 mg of pronuciferine (96.8% purity), 8.5 mg of nuciferine (98.9% purity), and 2.7 mg of roemerine (97.4%) respectively. The chemical structure of four aporphine alkaloids are identified by means of electrospray ionization MS (ESI-MS) and nuclear magnetic resonance (NMR) analysis. Moreover, the effects of four separated aporphine alkaloids on insulin-stimulated glucose consumption were examined in 3T3-L1 adipocytes. The results showed that 2-hydroxy-1-methoxyaporphine and pronuciferine increased the glucose consumption significantly as rosiglitazone did.; Aporphine alkaloids from the leaves of Nelumbo nucifera Gaertn are substances of great interest because of their important pharmacological activities, particularly anti-diabetic, anti-obesity, anti-hyperlipidemic, anti-oxidant, and anti-HIV's activities. In order to produce large amounts of pure alkaloid for research purposes, a novel method using high-speed counter-current chromatography (HSCCC) was developed. Without any initial cleanup steps, four main aporphine alkaloids, including 2-hydroxy-1-methoxyaporphine, pronuciferine, nuciferine and roemerine were successfully purified from the crude extract by HSCCC in one step. The separation was performed with a simple two-phase solvent system composed of n-hexane-ethyl acetate-methanol-acetonitrile-water (5:3:3:2.5:5, v/v/v/v/v). In each operation, 100 mg crude extracts was separated and yielded 6.3 mg of 2-hydroxy-1-methoxyaporphine (95.1% purity), 1.1 mg of pronuciferine (96.8% purity), 8.5 mg of nuciferine (98.9% purity), and 2.7 mg of roemerine (97.4%) respectively. The chemical structure of four aporphine alkaloids are identified by means of electrospray ionization MS (ESI-MS) and nuclear magnetic resonance (NMR) analysis. Moreover, the effects of four separated aporphine alkaloids on insulin-stimulated glucose consumption were examined in 3T3-L1 adipocytes. The results showed that 2-hydroxy-1-methoxyaporphine and pronuciferine increased the glucose consumption significantly as rosiglitazone did. |
关键词 | Nelumbo Nucifera Gaertn Aporphine Alkaloids High-speed Counter-current Chromatography (Hsccc) Isolation 3t3-l1 Adipocytes Glucose Consumption |
WOS标题词 | Science & Technology ; Physical Sciences |
关键词[WOS] | COUNTER-CURRENT CHROMATOGRAPHY ; CONSTITUENTS ; BOLDINE ; CELLS ; MICE ; BENZYLISOQUINOLINE ; ADIPONECTIN ; METABOLITES ; EXTRACT ; RATS |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Multidisciplinary |
WOS记录号 | WOS:000334444700006 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/4246 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Yantai Univ, Sch Life Sci, Yantai 264005, Peoples R China 2.Yantai Univ, Sch Pharm, Yantai 264005, Peoples R China 3.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining 810001, Peoples R China |
推荐引用方式 GB/T 7714 | Ma, Chengjun,Wang, Jinjun,Chu, Hongmei,et al. Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2014,15(3):3481-3494. |
APA | Ma, Chengjun.,Wang, Jinjun.,Chu, Hongmei.,Zhang, Xiaoxiao.,Wang, Zhenhua.,...&Li, Gang.(2014).Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,15(3),3481-3494. |
MLA | Ma, Chengjun,et al."Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 15.3(2014):3481-3494. |
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