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Characterization of y-type high-molecular-weight glutenins in tetraploid species of Leymus
Sun, Yanqi1; Pu, Zongjun2; Dai, Shoufen1; Pu, Xiaoxue1; Liu, Dengcai1,3; Wu, Bihua1; Lan, Xiujin1; Wei, Yuming1; Zheng, Youliang1; Yan, Zehong1
2014-02-01
Source PublicationDEVELOPMENT GENES AND EVOLUTION
ISSN0949-944X
Volume224Issue:1Pages:57-64
SubtypeArticle
AbstractThree y-type high-molecular-weight (HMW) glutenin gene open reading frames (ORFs), Chiy1, Chiy2, and Racy, were isolated and characterized from Leymus chinensis PI499516 and Leymus racemosus ssp. racemosus W623305. They shared an extra glutamine in the N-terminal and LAAQLPAMCRL peptides in the C-terminal with x-type HMW glutenins but had different N-terminal lengths. Like other y-type HMW glutenins, Chiy2 and Racy had 104 (or 105) amino acid (aa) residues at the N-terminal and started with EGEASR, whereas Chiy1 had 99 aa in this domain and started with QLQCER because of the deletion of EGEASR. Five other y-type glutenins, including those from Elymus ciliaris, Pseudoroegneria libanotica, and Leymus mollis, were similar to Chiy1. The ORF of Chiy2 was probably not expressed. The ORFs of both Chiy1 and Racy were expressed in bacteria. The maximum likelihood phylogenic tree based on the signal peptide and N-terminal and C-terminal aa residues revealed two clades of y-type HMW glutenins in Triticeae; the first contained Ay, By, Cy, Dy, Eey, Gy, Ky, Ry, Tay, and Uy, while the second clade contained the remaining y types, including those from Leymus. Within the second clade, HMW glutenins lacking the EGEASR peptide formed a subclade. These y-type HMW glutenins in Leymus could not be targeted to the Xm or Ns genome.; Three y-type high-molecular-weight (HMW) glutenin gene open reading frames (ORFs), Chiy1, Chiy2, and Racy, were isolated and characterized from Leymus chinensis PI499516 and Leymus racemosus ssp. racemosus W623305. They shared an extra glutamine in the N-terminal and LAAQLPAMCRL peptides in the C-terminal with x-type HMW glutenins but had different N-terminal lengths. Like other y-type HMW glutenins, Chiy2 and Racy had 104 (or 105) amino acid (aa) residues at the N-terminal and started with EGEASR, whereas Chiy1 had 99 aa in this domain and started with QLQCER because of the deletion of EGEASR. Five other y-type glutenins, including those from Elymus ciliaris, Pseudoroegneria libanotica, and Leymus mollis, were similar to Chiy1. The ORF of Chiy2 was probably not expressed. The ORFs of both Chiy1 and Racy were expressed in bacteria. The maximum likelihood phylogenic tree based on the signal peptide and N-terminal and C-terminal aa residues revealed two clades of y-type HMW glutenins in Triticeae; the first contained Ay, By, Cy, Dy, Eey, Gy, Ky, Ry, Tay, and Uy, while the second clade contained the remaining y types, including those from Leymus. Within the second clade, HMW glutenins lacking the EGEASR peptide formed a subclade. These y-type HMW glutenins in Leymus could not be targeted to the Xm or Ns genome.
KeywordGene Sequencing Glu-1 Leymus Nsxm Genome
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS KeywordSUBUNIT GENES ; SEQUENCES ; GENOME
Indexed BySCI
Language英语
WOS Research AreaCell Biology ; Evolutionary Biology ; Developmental Biology
WOS SubjectCell Biology ; Evolutionary Biology ; Developmental Biology
WOS IDWOS:000330719000006
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/4256
Collection中国科学院西北高原生物研究所
Affiliation1.Sichuan Agr Univ, Triticeae Res Inst, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Sichuan, Peoples R China
3.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China
Recommended Citation
GB/T 7714
Sun, Yanqi,Pu, Zongjun,Dai, Shoufen,et al. Characterization of y-type high-molecular-weight glutenins in tetraploid species of Leymus[J]. DEVELOPMENT GENES AND EVOLUTION,2014,224(1):57-64.
APA Sun, Yanqi.,Pu, Zongjun.,Dai, Shoufen.,Pu, Xiaoxue.,Liu, Dengcai.,...&Yan, Zehong.(2014).Characterization of y-type high-molecular-weight glutenins in tetraploid species of Leymus.DEVELOPMENT GENES AND EVOLUTION,224(1),57-64.
MLA Sun, Yanqi,et al."Characterization of y-type high-molecular-weight glutenins in tetraploid species of Leymus".DEVELOPMENT GENES AND EVOLUTION 224.1(2014):57-64.
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