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Characterization of y-type high-molecular-weight glutenins in tetraploid species of Leymus
Sun, Yanqi1; Pu, Zongjun2; Dai, Shoufen1; Pu, Xiaoxue1; Liu, Dengcai1,3; Wu, Bihua1; Lan, Xiujin1; Wei, Yuming1; Zheng, Youliang1; Yan, Zehong1
2014-02-01
发表期刊DEVELOPMENT GENES AND EVOLUTION
ISSN0949-944X
卷号224期号:1页码:57-64
文章类型Article
摘要Three y-type high-molecular-weight (HMW) glutenin gene open reading frames (ORFs), Chiy1, Chiy2, and Racy, were isolated and characterized from Leymus chinensis PI499516 and Leymus racemosus ssp. racemosus W623305. They shared an extra glutamine in the N-terminal and LAAQLPAMCRL peptides in the C-terminal with x-type HMW glutenins but had different N-terminal lengths. Like other y-type HMW glutenins, Chiy2 and Racy had 104 (or 105) amino acid (aa) residues at the N-terminal and started with EGEASR, whereas Chiy1 had 99 aa in this domain and started with QLQCER because of the deletion of EGEASR. Five other y-type glutenins, including those from Elymus ciliaris, Pseudoroegneria libanotica, and Leymus mollis, were similar to Chiy1. The ORF of Chiy2 was probably not expressed. The ORFs of both Chiy1 and Racy were expressed in bacteria. The maximum likelihood phylogenic tree based on the signal peptide and N-terminal and C-terminal aa residues revealed two clades of y-type HMW glutenins in Triticeae; the first contained Ay, By, Cy, Dy, Eey, Gy, Ky, Ry, Tay, and Uy, while the second clade contained the remaining y types, including those from Leymus. Within the second clade, HMW glutenins lacking the EGEASR peptide formed a subclade. These y-type HMW glutenins in Leymus could not be targeted to the Xm or Ns genome.; Three y-type high-molecular-weight (HMW) glutenin gene open reading frames (ORFs), Chiy1, Chiy2, and Racy, were isolated and characterized from Leymus chinensis PI499516 and Leymus racemosus ssp. racemosus W623305. They shared an extra glutamine in the N-terminal and LAAQLPAMCRL peptides in the C-terminal with x-type HMW glutenins but had different N-terminal lengths. Like other y-type HMW glutenins, Chiy2 and Racy had 104 (or 105) amino acid (aa) residues at the N-terminal and started with EGEASR, whereas Chiy1 had 99 aa in this domain and started with QLQCER because of the deletion of EGEASR. Five other y-type glutenins, including those from Elymus ciliaris, Pseudoroegneria libanotica, and Leymus mollis, were similar to Chiy1. The ORF of Chiy2 was probably not expressed. The ORFs of both Chiy1 and Racy were expressed in bacteria. The maximum likelihood phylogenic tree based on the signal peptide and N-terminal and C-terminal aa residues revealed two clades of y-type HMW glutenins in Triticeae; the first contained Ay, By, Cy, Dy, Eey, Gy, Ky, Ry, Tay, and Uy, while the second clade contained the remaining y types, including those from Leymus. Within the second clade, HMW glutenins lacking the EGEASR peptide formed a subclade. These y-type HMW glutenins in Leymus could not be targeted to the Xm or Ns genome.
关键词Gene Sequencing Glu-1 Leymus Nsxm Genome
WOS标题词Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]SUBUNIT GENES ; SEQUENCES ; GENOME
收录类别SCI
语种英语
WOS研究方向Cell Biology ; Evolutionary Biology ; Developmental Biology
WOS类目Cell Biology ; Evolutionary Biology ; Developmental Biology
WOS记录号WOS:000330719000006
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/4256
专题中国科学院西北高原生物研究所
作者单位1.Sichuan Agr Univ, Triticeae Res Inst, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Sichuan, Peoples R China
3.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China
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GB/T 7714
Sun, Yanqi,Pu, Zongjun,Dai, Shoufen,et al. Characterization of y-type high-molecular-weight glutenins in tetraploid species of Leymus[J]. DEVELOPMENT GENES AND EVOLUTION,2014,224(1):57-64.
APA Sun, Yanqi.,Pu, Zongjun.,Dai, Shoufen.,Pu, Xiaoxue.,Liu, Dengcai.,...&Yan, Zehong.(2014).Characterization of y-type high-molecular-weight glutenins in tetraploid species of Leymus.DEVELOPMENT GENES AND EVOLUTION,224(1),57-64.
MLA Sun, Yanqi,et al."Characterization of y-type high-molecular-weight glutenins in tetraploid species of Leymus".DEVELOPMENT GENES AND EVOLUTION 224.1(2014):57-64.
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