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SEPARATION AND PURIFICATION OF THREE STILBENES FROM THE RADIX OF Polygonum cillinerve (Nakai) Ohwl BY MACROPOROUS RESIN COLUMN CHROMATOGRAPHY COMBINED WITH HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY
Chi, Xiaofeng; Xing, Yuxiu; Xiao, Yuancan; Dong, Qi; Hu, Fengzu
2014
Source PublicationQUIMICA NOVA
ISSN0100-4042
Volume37Issue:9Pages:1465-1468
SubtypeArticle
AbstractAn effective method for the rapid separation and purification of three stilbenes from the radix of Polygonum cillinerve (Nakai) Ohwl by macroporous resin column chromatography combined with high-speed counter-current chromatography (HSCCC) was successfully established. In the present study, a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:1:4:2, v/v/v/v) was used for HSCCC separation. A one-step separation in 4 h from 150 mg of crude extract produced 26.3 mg of trans-resveratrol-3-O-glucoside, 42.0 mg of pieceid-2 ''-O-gallate, and 17.9 mg of trans-resveratrol with purities of 99.1%, 97.8%, and 99.4%, respectively, as determined by high-performance liquid chromatography (HPLC). The chemical structures of these compounds were identified by nuclear magnetic resonance (NMR) spectroscopy.; An effective method for the rapid separation and purification of three stilbenes from the radix of Polygonum cillinerve (Nakai) Ohwl by macroporous resin column chromatography combined with high-speed counter-current chromatography (HSCCC) was successfully established. In the present study, a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:1:4:2, v/v/v/v) was used for HSCCC separation. A one-step separation in 4 h from 150 mg of crude extract produced 26.3 mg of trans-resveratrol-3-O-glucoside, 42.0 mg of pieceid-2 ''-O-gallate, and 17.9 mg of trans-resveratrol with purities of 99.1%, 97.8%, and 99.4%, respectively, as determined by high-performance liquid chromatography (HPLC). The chemical structures of these compounds were identified by nuclear magnetic resonance (NMR) spectroscopy.
KeywordPolygonum Cillinerve (Nakai) Ohwl Hsccc Stilbenes
WOS HeadingsScience & Technology ; Physical Sciences
WOS KeywordVINIFERA CELL-CULTURES ; SUPERCRITICAL-FLUID EXTRACTION ; VITIS-VINIFERA ; PREPARATIVE SEPARATION ; ANTIOXIDANT ACTIVITY ; CUSPIDATUM ; LIGNANS ; PICEID
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000344472500007
Citation statistics
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/4264
Collection中国科学院西北高原生物研究所
AffiliationChinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China
Recommended Citation
GB/T 7714
Chi, Xiaofeng,Xing, Yuxiu,Xiao, Yuancan,et al. SEPARATION AND PURIFICATION OF THREE STILBENES FROM THE RADIX OF Polygonum cillinerve (Nakai) Ohwl BY MACROPOROUS RESIN COLUMN CHROMATOGRAPHY COMBINED WITH HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY[J]. QUIMICA NOVA,2014,37(9):1465-1468.
APA Chi, Xiaofeng,Xing, Yuxiu,Xiao, Yuancan,Dong, Qi,&Hu, Fengzu.(2014).SEPARATION AND PURIFICATION OF THREE STILBENES FROM THE RADIX OF Polygonum cillinerve (Nakai) Ohwl BY MACROPOROUS RESIN COLUMN CHROMATOGRAPHY COMBINED WITH HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY.QUIMICA NOVA,37(9),1465-1468.
MLA Chi, Xiaofeng,et al."SEPARATION AND PURIFICATION OF THREE STILBENES FROM THE RADIX OF Polygonum cillinerve (Nakai) Ohwl BY MACROPOROUS RESIN COLUMN CHROMATOGRAPHY COMBINED WITH HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY".QUIMICA NOVA 37.9(2014):1465-1468.
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